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- W1966289669 abstract "A two-step procedure is described in this paper to identify the position of O-acetyl groups in S-657 polysaccharide. Reductive-cleavage experiments performed on the fully methylated (base-catalyzed) polysaccharide, followed by acetylation of anhydroalditols, identified individual sugar residues and their position of linkage. In a second experiment, the polysaccharide was methylated under neutral conditions leaving native acetate groups intact. Reductive cleavage of the neutral methylated polysaccharide using CF3SO3SiMe3 as a catalyst, followed by acetylation in situ, identified sugar residues containing native acetate groups and established their position of substitution. Using this two-step procedure of analysis, S-657 polysaccharide is shown to contain O-acetyl groups on the 2-position and the 2,6-positions of 3-linked glucopyranosyl residues." @default.
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- W1966289669 date "2001-04-01" @default.
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- W1966289669 title "Location of O-acetyl groups in S-657 using the reductive-cleavage method" @default.
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- W1966289669 doi "https://doi.org/10.1016/s0008-6215(01)00045-3" @default.
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