FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966320246> ?p ?o ?g. }

• W1966320246 endingPage "120" @default.
• W1966320246 startingPage "111" @default.
• W1966320246 abstract "We examined the relationship between pre-mRNA splicing and the nuclear matrix by using an in vivo system that we have developed. Plasmids containing the inducible herpesvirus tk gene promoter linked to an intron-containing segment of the rabbit beta-globin gene were transfected into HeLa cells, and then the promoter was transactivated by infection with a TK- virus. Northern analysis revealed that the globin pre-mRNA and all its splicing intermediates and products are associated with the nuclear matrix prepared from such transfected cells. When the nuclear matrix was incubated with a HeLa cell in vitro splicing extract in the presence of ATP, the amount of matrix-associated precursor progressively decreased without a temporal lag in the reaction, with a corresponding increase in free intron lariat. Thus, most of the events of the splicing process (endonucleolytic cuts and branching) occur in this in vitro complementation reaction. However, ligation of exons cannot be monitored in this system because of the abundance of preexisting mature mRNA. Since the matrix is not a self-splicing entity, whereas the in vitro splicing system cannot process efficiently deproteinized matrix RNA, we conclude from our in vitro complementation results (which can be reproduced by using micrococcal nuclease-treated splicing extract) that the nuclear matrix preparation retains parts of preassembled ribonucleoprotein complexes that have the potential to function when supplemented with soluble factors (presumably other than most of the small nuclear ribonucleoproteins known to participate in splicing) present in the HeLa cell extract." @default.
• W1966320246 created "2016-06-24" @default.
• W1966320246 creator A5035758400 @default.
• W1966320246 creator A5036271984 @default.
• W1966320246 creator A5039952654 @default.
• W1966320246 creator A5048640524 @default.
• W1966320246 date "1987-01-01" @default.
• W1966320246 modified "2023-10-11" @default.
• W1966320246 title "Pre-mRNA splicing and the nuclear matrix." @default.
• W1966320246 cites W1476434754 @default.
• W1966320246 cites W1602474918 @default.
• W1966320246 cites W1820557301 @default.
• W1966320246 cites W1882271604 @default.
• W1966320246 cites W1884434891 @default.
• W1966320246 cites W1916525817 @default.
• W1966320246 cites W1968322556 @default.
• W1966320246 cites W1970010476 @default.
• W1966320246 cites W1973455868 @default.
• W1966320246 cites W1975642325 @default.
• W1966320246 cites W1986427478 @default.
• W1966320246 cites W1990823895 @default.
• W1966320246 cites W1993083297 @default.
• W1966320246 cites W1993243592 @default.
• W1966320246 cites W1994261379 @default.
• W1966320246 cites W1995805538 @default.
• W1966320246 cites W2000955810 @default.
• W1966320246 cites W2002944098 @default.
• W1966320246 cites W2008422577 @default.
• W1966320246 cites W2011335102 @default.
• W1966320246 cites W2024964870 @default.
• W1966320246 cites W2026073476 @default.
• W1966320246 cites W2039726375 @default.
• W1966320246 cites W2056391548 @default.
• W1966320246 cites W2060333964 @default.
• W1966320246 cites W2060948741 @default.
• W1966320246 cites W2065178657 @default.
• W1966320246 cites W2074427164 @default.
• W1966320246 cites W2084099638 @default.
• W1966320246 cites W2092377354 @default.
• W1966320246 cites W2108184942 @default.
• W1966320246 cites W2110671947 @default.
• W1966320246 cites W2119232680 @default.
• W1966320246 cites W2122071753 @default.
• W1966320246 cites W2125959590 @default.
• W1966320246 cites W2161524167 @default.
• W1966320246 cites W2167414261 @default.
• W1966320246 cites W2229886884 @default.
• W1966320246 cites W2418824759 @default.
• W1966320246 cites W3021597811 @default.
• W1966320246 cites W52144977 @default.
• W1966320246 doi "https://doi.org/10.1128/mcb.7.1.111" @default.
• W1966320246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/365047" @default.
• W1966320246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3031450" @default.
• W1966320246 hasPublicationYear "1987" @default.
• W1966320246 type Work @default.
• W1966320246 sameAs 1966320246 @default.
• W1966320246 citedByCount "198" @default.
• W1966320246 countsByYear W19663202462012 @default.
• W1966320246 countsByYear W19663202462013 @default.
• W1966320246 countsByYear W19663202462015 @default.
• W1966320246 countsByYear W19663202462016 @default.
• W1966320246 countsByYear W19663202462017 @default.
• W1966320246 countsByYear W19663202462019 @default.
• W1966320246 countsByYear W19663202462020 @default.
• W1966320246 countsByYear W19663202462021 @default.
• W1966320246 countsByYear W19663202462022 @default.
• W1966320246 countsByYear W19663202462023 @default.
• W1966320246 crossrefType "journal-article" @default.
• W1966320246 hasAuthorship W1966320246A5035758400 @default.
• W1966320246 hasAuthorship W1966320246A5036271984 @default.
• W1966320246 hasAuthorship W1966320246A5039952654 @default.
• W1966320246 hasAuthorship W1966320246A5048640524 @default.
• W1966320246 hasBestOaLocation W19663202462 @default.
• W1966320246 hasConcept C102063271 @default.
• W1966320246 hasConcept C104317684 @default.
• W1966320246 hasConcept C105686024 @default.
• W1966320246 hasConcept C153911025 @default.
• W1966320246 hasConcept C166342232 @default.
• W1966320246 hasConcept C2908584439 @default.
• W1966320246 hasConcept C36823959 @default.
• W1966320246 hasConcept C54355233 @default.
• W1966320246 hasConcept C54458228 @default.
• W1966320246 hasConcept C67705224 @default.
• W1966320246 hasConcept C75934600 @default.
• W1966320246 hasConcept C83640560 @default.
• W1966320246 hasConcept C86803240 @default.
• W1966320246 hasConcept C94671646 @default.
• W1966320246 hasConcept C95444343 @default.
• W1966320246 hasConceptScore W1966320246C102063271 @default.
• W1966320246 hasConceptScore W1966320246C104317684 @default.
• W1966320246 hasConceptScore W1966320246C105686024 @default.
• W1966320246 hasConceptScore W1966320246C153911025 @default.
• W1966320246 hasConceptScore W1966320246C166342232 @default.
• W1966320246 hasConceptScore W1966320246C2908584439 @default.
• W1966320246 hasConceptScore W1966320246C36823959 @default.
• W1966320246 hasConceptScore W1966320246C54355233 @default.
• W1966320246 hasConceptScore W1966320246C54458228 @default.
• W1966320246 hasConceptScore W1966320246C67705224 @default.

• next