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• W1966344544 endingPage "e71081" @default.
• W1966344544 startingPage "e71081" @default.
• W1966344544 abstract "During prion infection, the normal, protease-sensitive conformation of prion protein (PrPC) is converted via seeded polymerization to an abnormal, infectious conformation with greatly increased protease-resistance (PrPSc). In vitro, protein misfolding cyclic amplification (PMCA) uses PrPSc in prion-infected brain homogenates as an initiating seed to convert PrPC and trigger the self-propagation of PrPSc over many cycles of amplification. While PMCA reactions produce high levels of protease-resistant PrP, the infectious titer is often lower than that of brain-derived PrPSc. More recently, PMCA techniques using bacterially derived recombinant PrP (rPrP) in the presence of lipid and RNA but in the absence of any starting PrPSc seed have been used to generate infectious prions that cause disease in wild-type mice with relatively short incubation times. These data suggest that lipid and/or RNA act as cofactors to facilitate the de novo formation of high levels of prion infectivity. Using rPrP purified by two different techniques, we generated a self-propagating protease-resistant rPrP molecule that, regardless of the amount of RNA and lipid used, had a molecular mass, protease resistance and insolubility similar to that of PrPSc. However, we were unable to detect prion infectivity in any of our reactions using either cell-culture or animal bioassays. These results demonstrate that the ability to self-propagate into a protease-resistant insoluble conformer is not unique to infectious PrP molecules. They suggest that the presence of RNA and lipid cofactors may facilitate the spontaneous refolding of PrP into an infectious form while also allowing the de novo formation of self-propagating, but non-infectious, rPrP-res." @default.
• W1966344544 created "2016-06-24" @default.
• W1966344544 creator A5021192977 @default.
• W1966344544 creator A5036228242 @default.
• W1966344544 creator A5077072507 @default.
• W1966344544 creator A5084875829 @default.
• W1966344544 date "2013-07-30" @default.
• W1966344544 modified "2023-10-14" @default.
• W1966344544 title "Recombinant Prion Protein Refolded with Lipid and RNA Has the Biochemical Hallmarks of a Prion but Lacks In Vivo Infectivity" @default.
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• W1966344544 doi "https://doi.org/10.1371/journal.pone.0071081" @default.
• W1966344544 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3728029" @default.
• W1966344544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23936256" @default.
• W1966344544 hasPublicationYear "2013" @default.
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