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- W1966375553 abstract "A method is presented for measuring the molar extinction coefficient of free and protein-bound fluorescein-isothiocyanate. The molar extinction coefficient of the free dye is 1.18 times higher than that of the bound dye in a fluorescent conalbumin conjugate. The knowledge of the correct extinction coefficient is important for the accurate calculation of the fluorescein to protein ratio and the fluorescence quenching of fluorescent protein conjugates. The presence of serum proteins enhances the fluorescence of the conjugated protein, but decreases the fluorescence of the free dye." @default.
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- W1966375553 date "1966-09-01" @default.
- W1966375553 modified "2023-10-17" @default.
- W1966375553 title "Optical absorption and fluorescence of fluorescent protein conjugates" @default.
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- W1966375553 doi "https://doi.org/10.1016/0003-2697(66)90218-1" @default.
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