FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966384699> ?p ?o ?g. }

• W1966384699 endingPage "e1003922" @default.
• W1966384699 startingPage "e1003922" @default.
• W1966384699 abstract "Rift Valley fever virus (RVFV, family Bunyaviridae) is a mosquito-borne pathogen of both livestock and humans, found primarily in Sub-Saharan Africa and the Arabian Peninsula. The viral genome comprises two negative-sense (L and M segments) and one ambisense (S segment) RNAs that encode seven proteins. The S segment encodes the nucleocapsid (N) protein in the negative-sense and a nonstructural (NSs) protein in the positive-sense, though NSs cannot be translated directly from the S segment but rather from a specific subgenomic mRNA. Using reverse genetics we generated a virus, designated rMP12:S-Swap, in which the N protein is expressed from the NSs locus and NSs from the N locus within the genomic S RNA. In cells infected with rMP12:S-Swap NSs is expressed at higher levels with respect to N than in cells infected with the parental rMP12 virus. Despite NSs being the main interferon antagonist and determinant of virulence, growth of rMP12:S-Swap was attenuated in mammalian cells and gave a small plaque phenotype. The increased abundance of the NSs protein did not lead to faster inhibition of host cell protein synthesis or host cell transcription in infected mammalian cells. In cultured mosquito cells, however, infection with rMP12:S-Swap resulted in cell death rather than establishment of persistence as seen with rMP12. Finally, altering the composition of the S segment led to a differential packaging ratio of genomic to antigenomic RNA into rMP12:S-Swap virions. Our results highlight the plasticity of the RVFV genome and provide a useful experimental tool to investigate further the packaging mechanism of the segmented genome." @default.
• W1966384699 created "2016-06-24" @default.
• W1966384699 creator A5006227397 @default.
• W1966384699 creator A5012677136 @default.
• W1966384699 creator A5075639363 @default.
• W1966384699 date "2014-02-13" @default.
• W1966384699 modified "2023-10-04" @default.
• W1966384699 title "The Consequences of Reconfiguring the Ambisense S Genome Segment of Rift Valley Fever Virus on Viral Replication in Mammalian and Mosquito Cells and for Genome Packaging" @default.
• W1966384699 cites W1676678472 @default.
• W1966384699 cites W1686908682 @default.
• W1966384699 cites W1779527395 @default.
• W1966384699 cites W1964541411 @default.
• W1966384699 cites W1966385881 @default.
• W1966384699 cites W1981919527 @default.
• W1966384699 cites W1987438638 @default.
• W1966384699 cites W1999215269 @default.
• W1966384699 cites W2010970638 @default.
• W1966384699 cites W2014455751 @default.
• W1966384699 cites W2017602138 @default.
• W1966384699 cites W2020447874 @default.
• W1966384699 cites W2021618307 @default.
• W1966384699 cites W2023010917 @default.
• W1966384699 cites W2025106987 @default.
• W1966384699 cites W2027972306 @default.
• W1966384699 cites W2031050801 @default.
• W1966384699 cites W2032549397 @default.
• W1966384699 cites W2046937661 @default.
• W1966384699 cites W2048478321 @default.
• W1966384699 cites W2056450967 @default.
• W1966384699 cites W2057494472 @default.
• W1966384699 cites W2059537839 @default.
• W1966384699 cites W2061412928 @default.
• W1966384699 cites W2065511410 @default.
• W1966384699 cites W2068748104 @default.
• W1966384699 cites W2073253018 @default.
• W1966384699 cites W2077266113 @default.
• W1966384699 cites W2079670291 @default.
• W1966384699 cites W2081620517 @default.
• W1966384699 cites W2088335903 @default.
• W1966384699 cites W2090494535 @default.
• W1966384699 cites W2098951059 @default.
• W1966384699 cites W2099479563 @default.
• W1966384699 cites W2102722149 @default.
• W1966384699 cites W2118420574 @default.
• W1966384699 cites W2119458212 @default.
• W1966384699 cites W2125897056 @default.
• W1966384699 cites W2126812573 @default.
• W1966384699 cites W2134294929 @default.
• W1966384699 cites W2136817553 @default.
• W1966384699 cites W2141978871 @default.
• W1966384699 cites W2143497826 @default.
• W1966384699 cites W2143943017 @default.
• W1966384699 cites W2144199271 @default.
• W1966384699 cites W2146182603 @default.
• W1966384699 cites W2147060534 @default.
• W1966384699 cites W2149805957 @default.
• W1966384699 cites W2152651960 @default.
• W1966384699 cites W2156225853 @default.
• W1966384699 cites W2157873574 @default.
• W1966384699 cites W2159779332 @default.
• W1966384699 cites W2159900229 @default.
• W1966384699 cites W2166343612 @default.
• W1966384699 doi "https://doi.org/10.1371/journal.ppat.1003922" @default.
• W1966384699 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3923772" @default.
• W1966384699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24550727" @default.
• W1966384699 hasPublicationYear "2014" @default.
• W1966384699 type Work @default.
• W1966384699 sameAs 1966384699 @default.
• W1966384699 citedByCount "35" @default.
• W1966384699 countsByYear W19663846992014 @default.
• W1966384699 countsByYear W19663846992015 @default.
• W1966384699 countsByYear W19663846992016 @default.
• W1966384699 countsByYear W19663846992017 @default.
• W1966384699 countsByYear W19663846992018 @default.
• W1966384699 countsByYear W19663846992019 @default.
• W1966384699 countsByYear W19663846992021 @default.
• W1966384699 countsByYear W19663846992022 @default.
• W1966384699 countsByYear W19663846992023 @default.
• W1966384699 crossrefType "journal-article" @default.
• W1966384699 hasAuthorship W1966384699A5006227397 @default.
• W1966384699 hasAuthorship W1966384699A5012677136 @default.
• W1966384699 hasAuthorship W1966384699A5075639363 @default.
• W1966384699 hasBestOaLocation W19663846991 @default.
• W1966384699 hasConcept C104317684 @default.
• W1966384699 hasConcept C140704245 @default.
• W1966384699 hasConcept C141231307 @default.
• W1966384699 hasConcept C159047783 @default.
• W1966384699 hasConcept C189819185 @default.
• W1966384699 hasConcept C2522874641 @default.
• W1966384699 hasConcept C25365240 @default.
• W1966384699 hasConcept C2776178377 @default.
• W1966384699 hasConcept C2778852064 @default.
• W1966384699 hasConcept C54355233 @default.
• W1966384699 hasConcept C67705224 @default.
• W1966384699 hasConcept C86803240 @default.
• W1966384699 hasConceptScore W1966384699C104317684 @default.
• W1966384699 hasConceptScore W1966384699C140704245 @default.
• W1966384699 hasConceptScore W1966384699C141231307 @default.

• next