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- W1966385714 abstract "High-throughput DNA sequencing technologies are fuelling an accelerating trend to assemble de novo or resequence the genomes of numerous species as well as to complete unfinished assemblies. While current DNA sequencing technologies remain limited to reading stretches of a few hundreds or thousands of base pairs, experimental and computational methods are continuously improving with the goal of assembling entire genomes from large numbers of short DNA sequences. However, the algorithms that piece together DNA strands face important limitations due, notably, to the presence of repeated sequences or of multiple haplotypes within one genome, thus leaving many assemblies incomplete. Recently, the realization that the physical contacts experienced by a portion of a DNA molecule could be used as a robust and quantitative assay to determine its genomic position has led to the emerging field of contact genomics, which promises to revolutionize current genome assembly approaches by exploiting the flexible polymer properties of chromosomes. Here we review the current applications of contact genomics to genome scaffolding, haplotyping and metagenomic assembly, then outline the future developments we envision." @default.
- W1966385714 created "2016-06-24" @default.
- W1966385714 creator A5039947505 @default.
- W1966385714 creator A5045736877 @default.
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- W1966385714 date "2015-04-29" @default.
- W1966385714 modified "2023-09-25" @default.
- W1966385714 title "Contact genomics: scaffolding and phasing (meta)genomes using chromosome 3D physical signatures" @default.
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- W1966385714 doi "https://doi.org/10.1016/j.febslet.2015.04.034" @default.
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