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- W1966451601 abstract "We obtained two novel W chromosome-linked chick genes by the use of female-male subtraction macroarrays, one of which, 2d-2F9, (recorded as AB188527 in DDBJ) did not have sufficient length (776 bp) to reveal its real form or characteristics. Hence, we obtained full-length Z-linked and W-linked 2d-2F9 genes of 2596 bp and 2589 bp respectively by the oligo-capping and RACE methods. Sequence analysis of these genes not only revealed that there is a counterpart of the W-linked 2d-2F9 gene on the Z chromosome, but also that there is a low homologous area at 5'-UTR between the W- and Z-kinked genes. Using this information, we designed a set of primers to identify sex and to select clones having the Z and W-linked gene (named 2d-2F9-Z and 2d-2F9-W), and also prepared two sets of primers for RT-PCR. These genes were found to be expressed constitutively and ubiquitously from the early embryo to the hatched chick, and they were assigned to the AAA ATP-superfamily." @default.
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- W1966451601 date "2007-02-23" @default.
- W1966451601 modified "2023-10-14" @default.
- W1966451601 title "Sequence Analysis of Full-Length cDNA of Sex Chromosome-Linked Novel Gene 2d-2F9 inGallus gallus" @default.
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- W1966451601 doi "https://doi.org/10.1271/bbb.60611" @default.
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