FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966454949> ?p ?o ?g. }

• W1966454949 endingPage "422" @default.
• W1966454949 startingPage "413" @default.
• W1966454949 abstract "Deamination of aromatic amino acids phenylalanine or tyrosine is accomplished by the phenylalanine/tyrosine ammonia-lyase (PAL/TAL) enzyme. TAL enzyme is of interest since deamination of tyrosine produces p-hydroxycinnamic acid (pHCA), which has potential for a variety of applications. Among nine microorganisms tested for their ability to produce tyrosine-inducible TAL activity, the yeast, Trichosporon cutaneum showed the highest TAL catalytic activity and the lowest PAL/TAL catalytic efficiency ratio (0.8). The enzyme was purified to near homogeneity and its kinetics studied. The native enzyme appears to be a homo-tetramer with a calculated MW of 294 kDa, subunit MW of 73.5 kDa, and a pI of 5.8. When phenylalanine was used as substrate, the Vmax, Kcat and Km were ∼4.0 ± 0.2 μg/min/mg purified enzyme), 588 ± 29 per min and 4.9 ± 0.9 mM, respectively. However, when tyrosine served as the substrate the Vmax and Kcat were 0.59 ± 0.02 μg/min/mg purified enzyme), and 86.7 ± 29 per min, and substrate binding was apparently cooperative (nH ∼ 2.6 ± 0.4), with S0.5 ∼ 0.6 mM. This is the first reported positive cooperativity for a TAL enzyme. Based on the NH2-terminal and partial internal peptide sequences, the cDNA encoding the enzyme was cloned. Sequence analysis of TcTAL showed 56–62% similarity to other fungal PAL/TAL enzymes. High-level expression (∼30% of total soluble protein, based on SDS-PAGE analysis) of the cDNA in Escherichia coli was achieved using the arabinose inducible araB promoter. The recombinant enzyme possessed both PAL and TAL activities, as evident from the presence of both pHCA and CA in the culture medium." @default.
• W1966454949 created "2016-06-24" @default.
• W1966454949 creator A5003643497 @default.
• W1966454949 creator A5045123864 @default.
• W1966454949 creator A5050678468 @default.
• W1966454949 creator A5084572287 @default.
• W1966454949 creator A5085264064 @default.
• W1966454949 date "2007-09-01" @default.
• W1966454949 modified "2023-10-16" @default.
• W1966454949 title "Functional expression in Escherichia coli of the tyrosine-inducible tyrosine ammonia-lyase enzyme from yeast Trichosporon cutaneum for production of p-hydroxycinnamic acid" @default.
• W1966454949 cites W1497740959 @default.
• W1966454949 cites W1501516808 @default.
• W1966454949 cites W1546651842 @default.
• W1966454949 cites W193921617 @default.
• W1966454949 cites W1970447590 @default.
• W1966454949 cites W1970851071 @default.
• W1966454949 cites W1971662732 @default.
• W1966454949 cites W1971836016 @default.
• W1966454949 cites W1981957241 @default.
• W1966454949 cites W1997844215 @default.
• W1966454949 cites W2013718446 @default.
• W1966454949 cites W2017723937 @default.
• W1966454949 cites W2022598269 @default.
• W1966454949 cites W2027974946 @default.
• W1966454949 cites W2046208134 @default.
• W1966454949 cites W2048310309 @default.
• W1966454949 cites W2055043387 @default.
• W1966454949 cites W2055573691 @default.
• W1966454949 cites W2057578763 @default.
• W1966454949 cites W2073624514 @default.
• W1966454949 cites W2074581799 @default.
• W1966454949 cites W2077460306 @default.
• W1966454949 cites W2091255112 @default.
• W1966454949 cites W2108119743 @default.
• W1966454949 cites W2109538083 @default.
• W1966454949 cites W2113964697 @default.
• W1966454949 cites W2115495905 @default.
• W1966454949 cites W2119495364 @default.
• W1966454949 cites W2125087385 @default.
• W1966454949 cites W2138176666 @default.
• W1966454949 cites W2160050498 @default.
• W1966454949 cites W2214826665 @default.
• W1966454949 cites W2313506150 @default.
• W1966454949 cites W4293247451 @default.
• W1966454949 doi "https://doi.org/10.1016/j.enzmictec.2007.03.013" @default.
• W1966454949 hasPublicationYear "2007" @default.
• W1966454949 type Work @default.
• W1966454949 sameAs 1966454949 @default.
• W1966454949 citedByCount "36" @default.
• W1966454949 countsByYear W19664549492013 @default.
• W1966454949 countsByYear W19664549492014 @default.
• W1966454949 countsByYear W19664549492015 @default.
• W1966454949 countsByYear W19664549492016 @default.
• W1966454949 countsByYear W19664549492018 @default.
• W1966454949 countsByYear W19664549492019 @default.
• W1966454949 countsByYear W19664549492020 @default.
• W1966454949 countsByYear W19664549492021 @default.
• W1966454949 countsByYear W19664549492022 @default.
• W1966454949 countsByYear W19664549492023 @default.
• W1966454949 crossrefType "journal-article" @default.
• W1966454949 hasAuthorship W1966454949A5003643497 @default.
• W1966454949 hasAuthorship W1966454949A5045123864 @default.
• W1966454949 hasAuthorship W1966454949A5050678468 @default.
• W1966454949 hasAuthorship W1966454949A5084572287 @default.
• W1966454949 hasAuthorship W1966454949A5085264064 @default.
• W1966454949 hasConcept C145782189 @default.
• W1966454949 hasConcept C181199279 @default.
• W1966454949 hasConcept C185592680 @default.
• W1966454949 hasConcept C2776165026 @default.
• W1966454949 hasConcept C2777431362 @default.
• W1966454949 hasConcept C2778112864 @default.
• W1966454949 hasConcept C41183919 @default.
• W1966454949 hasConcept C515207424 @default.
• W1966454949 hasConcept C55493867 @default.
• W1966454949 hasConcept C56856141 @default.
• W1966454949 hasConcept C86803240 @default.
• W1966454949 hasConceptScore W1966454949C145782189 @default.
• W1966454949 hasConceptScore W1966454949C181199279 @default.
• W1966454949 hasConceptScore W1966454949C185592680 @default.
• W1966454949 hasConceptScore W1966454949C2776165026 @default.
• W1966454949 hasConceptScore W1966454949C2777431362 @default.
• W1966454949 hasConceptScore W1966454949C2778112864 @default.
• W1966454949 hasConceptScore W1966454949C41183919 @default.
• W1966454949 hasConceptScore W1966454949C515207424 @default.
• W1966454949 hasConceptScore W1966454949C55493867 @default.
• W1966454949 hasConceptScore W1966454949C56856141 @default.
• W1966454949 hasConceptScore W1966454949C86803240 @default.
• W1966454949 hasIssue "4" @default.
• W1966454949 hasLocation W19664549491 @default.
• W1966454949 hasOpenAccess W1966454949 @default.
• W1966454949 hasPrimaryLocation W19664549491 @default.
• W1966454949 hasRelatedWork W1513881935 @default.
• W1966454949 hasRelatedWork W1980936780 @default.
• W1966454949 hasRelatedWork W2057155383 @default.
• W1966454949 hasRelatedWork W2057410200 @default.
• W1966454949 hasRelatedWork W2060862331 @default.
• W1966454949 hasRelatedWork W2066530401 @default.
• W1966454949 hasRelatedWork W2095561130 @default.

• next