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• W1966462625 abstract "Flow cytometry is a well-established and powerful high- throughput fluorescence measurement tool that also allows for the sorting and enrichment of subpopulations of cells expressing unique fluorescence signatures. Owing to the reliance on intensity-only signals, flow cytometry sorters cannot easily discriminate between fluorophores that spectrally overlap. In this paper we demonstrate a new method of cell sorting using a fluorescence lifetime-dependent methodology. This approach, referred to herein as phase-filtered cell sorting (PFCS), permits sorting based on the average fluorescence lifetime of a fluorophore by separating fluorescence signals from species that emit differing average fluorescence lifetimes. Using lifetime-dependent hardware, cells and microspheres labeled with fluorophores were sorted with purities up to 90%. PFCS is a practical approach for separating populations of cells that are stained with spectrally overlapping fluorophores or that have interfering autofluorescence signals." @default.
• W1966462625 created "2016-06-24" @default.
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• W1966462625 date "2013-06-14" @default.
• W1966462625 modified "2023-09-26" @default.
• W1966462625 title "Cytometric sorting based on the fluorescence lifetime of spectrally overlapping signals" @default.
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• W1966462625 doi "https://doi.org/10.1364/oe.21.014816" @default.
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