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- W1966523797 abstract "Abstract Proteolytic activity of some milk-clotting enzymes [calf rennet (chymosin), Mucor miehei rennet, and Cryphonectria parasitica rennet] on κ-casein was determined with reverse-phase HPLC. All enzymes were standardized to the same milk-clotting activity and incubated with κ-casein at 37°C for 1, 5, 15, 30, and 60min. Protein hydrolysis was stopped by addition of 10 –5 M pepstatin in 8 M urea. Chromatograms of hydrolysis products revealed that the enzymes hydrolyzed κ-casein differently. Chymosin hydrolysis was limited to formation of κ-macropeptide and para-κ-casein; the microbial rennets, particularly that from M. miehei , effected extensive nonspecific hydrolysis of both κ-casein and para-κ-casein." @default.
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- W1966523797 date "1992-06-01" @default.
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- W1966523797 title "Proteolytic Activity of Some Milk-Clotting Enzymes on κ-Casein" @default.
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- W1966523797 doi "https://doi.org/10.3168/jds.s0022-0302(92)77889-8" @default.
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