FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966535636> ?p ?o ?g. }

• W1966535636 endingPage "718" @default.
• W1966535636 startingPage "704" @default.
• W1966535636 abstract "Since most antibodies directed against protein antigens recognize epitopes composed of several discontinuous segments of the polypeptide chain, attempts to delineate the amino acids constituting these epitopes with the use of linear peptides have generally been unsuccessful. Here, a method is described based on error-prone PCR, phage display and negative selection, whereby amino acid residues constituting the functional epitope are identified in the context of the native protein. First a library of randomized antigen variants containing most single, double and triple amino acid mutants generated by single nucleotide substitutions is produced by error-prone PCR amplification of the DNA sequence encoding the protein antigen. The phage-displayed library is then negatively selected for epitope loss mutants by passing through an affinity matrix derivatized with a specific antibody and positively selected for retention of function. This method was applied to the mapping of the epitopes of two murine monoclonal antibodies (MA-7H11 and MA-3G10) on staphylokinase, a 136 amino acid plasminogen activator secreted by some strains of Staphylococcus aureus. After two negative/positive selection cycles, DNA sequencing of several clones revealed preferential amino acid mutations at positions 35 and 130 (with MA-7H11), and at positions 62, 66 and 136 (with MA-3G10). Affinity measurements of staphylokinase variants carrying single amino acid mutations at these positions confirmed their contribution to the free energy of binding to MA-7H11 and MA-3G10. This approach may be useful for isolating mutants with altered antigenic or functional properties and in general to map critical regions for protein-protein interactions." @default.
• W1966535636 created "2016-06-24" @default.
• W1966535636 creator A5013144378 @default.
• W1966535636 creator A5014998575 @default.
• W1966535636 creator A5015042160 @default.
• W1966535636 creator A5023982501 @default.
• W1966535636 date "1997-07-01" @default.
• W1966535636 modified "2023-10-13" @default.
• W1966535636 title "Epitope mapping by negative selection of randomized antigen libraries displayed on filamentous phage 1 1Edited by J. Karn" @default.
• W1966535636 cites W127614734 @default.
• W1966535636 cites W1509772502 @default.
• W1966535636 cites W1537376484 @default.
• W1966535636 cites W1549258100 @default.
• W1966535636 cites W1607323180 @default.
• W1966535636 cites W1625079248 @default.
• W1966535636 cites W1886152626 @default.
• W1966535636 cites W1974166931 @default.
• W1966535636 cites W1994501723 @default.
• W1966535636 cites W1996740379 @default.
• W1966535636 cites W1998591058 @default.
• W1966535636 cites W1999271199 @default.
• W1966535636 cites W1999383345 @default.
• W1966535636 cites W2005707246 @default.
• W1966535636 cites W2009910931 @default.
• W1966535636 cites W2010649076 @default.
• W1966535636 cites W2021915803 @default.
• W1966535636 cites W2025827008 @default.
• W1966535636 cites W2032806769 @default.
• W1966535636 cites W2034639222 @default.
• W1966535636 cites W2036079529 @default.
• W1966535636 cites W2051184695 @default.
• W1966535636 cites W2051750973 @default.
• W1966535636 cites W2062599442 @default.
• W1966535636 cites W2066386270 @default.
• W1966535636 cites W2071086763 @default.
• W1966535636 cites W2080032720 @default.
• W1966535636 cites W2086107215 @default.
• W1966535636 cites W2088031074 @default.
• W1966535636 cites W2104394178 @default.
• W1966535636 cites W2113530505 @default.
• W1966535636 cites W2136701955 @default.
• W1966535636 cites W2138270253 @default.
• W1966535636 cites W2149464124 @default.
• W1966535636 cites W2152845055 @default.
• W1966535636 cites W2155309949 @default.
• W1966535636 cites W2163591430 @default.
• W1966535636 cites W2319164130 @default.
• W1966535636 cites W3016224201 @default.
• W1966535636 cites W4256315477 @default.
• W1966535636 cites W4293247451 @default.
• W1966535636 doi "https://doi.org/10.1006/jmbi.1997.1077" @default.
• W1966535636 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9223635" @default.
• W1966535636 hasPublicationYear "1997" @default.
• W1966535636 type Work @default.
• W1966535636 sameAs 1966535636 @default.
• W1966535636 citedByCount "24" @default.
• W1966535636 countsByYear W19665356362014 @default.
• W1966535636 countsByYear W19665356362019 @default.
• W1966535636 countsByYear W19665356362022 @default.
• W1966535636 crossrefType "journal-article" @default.
• W1966535636 hasAuthorship W1966535636A5013144378 @default.
• W1966535636 hasAuthorship W1966535636A5014998575 @default.
• W1966535636 hasAuthorship W1966535636A5015042160 @default.
• W1966535636 hasAuthorship W1966535636A5023982501 @default.
• W1966535636 hasConcept C104317684 @default.
• W1966535636 hasConcept C143065580 @default.
• W1966535636 hasConcept C147483822 @default.
• W1966535636 hasConcept C153911025 @default.
• W1966535636 hasConcept C159654299 @default.
• W1966535636 hasConcept C167625842 @default.
• W1966535636 hasConcept C186268636 @default.
• W1966535636 hasConcept C195616568 @default.
• W1966535636 hasConcept C2781241564 @default.
• W1966535636 hasConcept C515207424 @default.
• W1966535636 hasConcept C542903549 @default.
• W1966535636 hasConcept C54355233 @default.
• W1966535636 hasConcept C55493867 @default.
• W1966535636 hasConcept C78598654 @default.
• W1966535636 hasConcept C86803240 @default.
• W1966535636 hasConceptScore W1966535636C104317684 @default.
• W1966535636 hasConceptScore W1966535636C143065580 @default.
• W1966535636 hasConceptScore W1966535636C147483822 @default.
• W1966535636 hasConceptScore W1966535636C153911025 @default.
• W1966535636 hasConceptScore W1966535636C159654299 @default.
• W1966535636 hasConceptScore W1966535636C167625842 @default.
• W1966535636 hasConceptScore W1966535636C186268636 @default.
• W1966535636 hasConceptScore W1966535636C195616568 @default.
• W1966535636 hasConceptScore W1966535636C2781241564 @default.
• W1966535636 hasConceptScore W1966535636C515207424 @default.
• W1966535636 hasConceptScore W1966535636C542903549 @default.
• W1966535636 hasConceptScore W1966535636C54355233 @default.
• W1966535636 hasConceptScore W1966535636C55493867 @default.
• W1966535636 hasConceptScore W1966535636C78598654 @default.
• W1966535636 hasConceptScore W1966535636C86803240 @default.
• W1966535636 hasIssue "5" @default.
• W1966535636 hasLocation W19665356361 @default.
• W1966535636 hasLocation W19665356362 @default.
• W1966535636 hasOpenAccess W1966535636 @default.

• next