Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966548345> ?p ?o ?g. }
- W1966548345 endingPage "92" @default.
- W1966548345 startingPage "85" @default.
- W1966548345 abstract "The drug-metabolizing enzyme thiopurine S-methyltransferase (TPMT) catalyzes the S-methylation of thiopurines such as 6-mercaptopurine, 6-thioguanine, and azathiopurine, which are used as immunosuppressants and in the treatment of acute lymphoblastic leukemia and rheumatoid arthritis. TPMT enzymatic activity is a polymorphic trait, and poor metabolizers may develop life-threatening bone marrow failure. To avoid such adverse effects, the TPMT enzymatic activity in patients' red blood cells (RBCs) is routinely measured prior to thiopurine administration in a limited number of oncology clinics. In the present study, we took advantage of a highly sensitive and specific automated denaturing high-performance liquid chromatography (dHPLC) technique that not only detects known polymorphic alleles, but also identifies previously uncharacterized sequence variants. We developed a dHPLC-based protocol to analyze the entire coding region and validated the protocol to detect all 16 previously described variant alleles. We further analyzed the entire coding region of the TPMT gene in 288 control samples collected worldwide and identified two novel amino acid substitutions Arg163Cys (487C>T) and Arg226Gln (677G>A) within exons 7 and 10, respectively. The clinical application of this comprehensive screening system for examining the entire TPMT gene would help to identify patients at risk for bone marrow failure prior to 6-mercaptopurine therapy." @default.
- W1966548345 created "2016-06-24" @default.
- W1966548345 creator A5002397415 @default.
- W1966548345 creator A5026657616 @default.
- W1966548345 creator A5026794757 @default.
- W1966548345 creator A5034413578 @default.
- W1966548345 creator A5037744919 @default.
- W1966548345 creator A5049591594 @default.
- W1966548345 creator A5064646764 @default.
- W1966548345 creator A5074812415 @default.
- W1966548345 creator A5088798513 @default.
- W1966548345 date "2005-06-01" @default.
- W1966548345 modified "2023-09-26" @default.
- W1966548345 title "Comprehensive Screening of the Thiopurine Methyltransferase Polymorphisms by Denaturing High-Performance Liquid Chromatography" @default.
- W1966548345 cites W1534517380 @default.
- W1966548345 cites W1546157935 @default.
- W1966548345 cites W1574225715 @default.
- W1966548345 cites W1893186719 @default.
- W1966548345 cites W1972773868 @default.
- W1966548345 cites W1978270674 @default.
- W1966548345 cites W1978999706 @default.
- W1966548345 cites W1984169395 @default.
- W1966548345 cites W1996409190 @default.
- W1966548345 cites W2004962018 @default.
- W1966548345 cites W2009663932 @default.
- W1966548345 cites W2016756549 @default.
- W1966548345 cites W2027950584 @default.
- W1966548345 cites W2029841402 @default.
- W1966548345 cites W2060310903 @default.
- W1966548345 cites W2061764105 @default.
- W1966548345 cites W2063213712 @default.
- W1966548345 cites W2075888728 @default.
- W1966548345 cites W2081422634 @default.
- W1966548345 cites W2081749017 @default.
- W1966548345 cites W2085038188 @default.
- W1966548345 cites W2090715680 @default.
- W1966548345 cites W2092002183 @default.
- W1966548345 cites W2116765834 @default.
- W1966548345 cites W2135506468 @default.
- W1966548345 cites W2148588489 @default.
- W1966548345 cites W2153127576 @default.
- W1966548345 cites W2181469541 @default.
- W1966548345 cites W2335253526 @default.
- W1966548345 cites W2337433694 @default.
- W1966548345 cites W2409149399 @default.
- W1966548345 cites W2441800335 @default.
- W1966548345 cites W2952849341 @default.
- W1966548345 cites W30502869 @default.
- W1966548345 doi "https://doi.org/10.1089/gte.2005.9.85" @default.
- W1966548345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15943548" @default.
- W1966548345 hasPublicationYear "2005" @default.
- W1966548345 type Work @default.
- W1966548345 sameAs 1966548345 @default.
- W1966548345 citedByCount "8" @default.
- W1966548345 countsByYear W19665483452014 @default.
- W1966548345 countsByYear W19665483452016 @default.
- W1966548345 crossrefType "journal-article" @default.
- W1966548345 hasAuthorship W1966548345A5002397415 @default.
- W1966548345 hasAuthorship W1966548345A5026657616 @default.
- W1966548345 hasAuthorship W1966548345A5026794757 @default.
- W1966548345 hasAuthorship W1966548345A5034413578 @default.
- W1966548345 hasAuthorship W1966548345A5037744919 @default.
- W1966548345 hasAuthorship W1966548345A5049591594 @default.
- W1966548345 hasAuthorship W1966548345A5064646764 @default.
- W1966548345 hasAuthorship W1966548345A5074812415 @default.
- W1966548345 hasAuthorship W1966548345A5088798513 @default.
- W1966548345 hasConcept C104317684 @default.
- W1966548345 hasConcept C126322002 @default.
- W1966548345 hasConcept C203014093 @default.
- W1966548345 hasConcept C2776053618 @default.
- W1966548345 hasConcept C2776760755 @default.
- W1966548345 hasConcept C2777173762 @default.
- W1966548345 hasConcept C2779134260 @default.
- W1966548345 hasConcept C2780007613 @default.
- W1966548345 hasConcept C36823959 @default.
- W1966548345 hasConcept C54355233 @default.
- W1966548345 hasConcept C70917649 @default.
- W1966548345 hasConcept C71924100 @default.
- W1966548345 hasConcept C86803240 @default.
- W1966548345 hasConcept C98274493 @default.
- W1966548345 hasConceptScore W1966548345C104317684 @default.
- W1966548345 hasConceptScore W1966548345C126322002 @default.
- W1966548345 hasConceptScore W1966548345C203014093 @default.
- W1966548345 hasConceptScore W1966548345C2776053618 @default.
- W1966548345 hasConceptScore W1966548345C2776760755 @default.
- W1966548345 hasConceptScore W1966548345C2777173762 @default.
- W1966548345 hasConceptScore W1966548345C2779134260 @default.
- W1966548345 hasConceptScore W1966548345C2780007613 @default.
- W1966548345 hasConceptScore W1966548345C36823959 @default.
- W1966548345 hasConceptScore W1966548345C54355233 @default.
- W1966548345 hasConceptScore W1966548345C70917649 @default.
- W1966548345 hasConceptScore W1966548345C71924100 @default.
- W1966548345 hasConceptScore W1966548345C86803240 @default.
- W1966548345 hasConceptScore W1966548345C98274493 @default.
- W1966548345 hasIssue "2" @default.
- W1966548345 hasLocation W19665483451 @default.
- W1966548345 hasLocation W19665483452 @default.
- W1966548345 hasOpenAccess W1966548345 @default.