FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966551301> ?p ?o ?g. }

• W1966551301 endingPage "8632" @default.
• W1966551301 startingPage "8620" @default.
• W1966551301 abstract "The YlqF/YawG families are important GTPases involved in ribosome biogenesis, cell proliferation, or cell growth, however, no plant homologs have yet to be characterized. Here we isolated rice (Oryza sativa) and Arabidopsis nuclear/nucleolar GTPase 2 (OsNug2 and AtNug2, respectively) that belong to the YawG subfamily and characterized them for pre-60S ribosomal subunit maturation. They showed typical intrinsic YlqF/YawG family GTPase activities in bacteria and yeasts with k(cat) values 0.12 ± 0.007 min(-1) (n = 6) and 0.087 ± 0.002 min(-1) (n = 4), respectively, and addition of 60S ribosomal subunits stimulated their activities in vitro. In addition, OsNug2 rescued the lethality of the yeast nug2 null mutant through recovery of 25S pre-rRNA processing. By yeast two-hybrid screening five clones, including a putative one of 60S ribosomal proteins, OsL10a, were isolated. Subcellular localization and pulldown assays resulted in that the N-terminal region of OsNug2 is sufficient for nucleolar/nuclear targeting and association with OsL10a. OsNug2 is physically associated with pre-60S ribosomal complexes highly enriched in the 25S, 5.8S, and 5S rRNA, and its interaction was stimulated by exogenous GTP. Furthermore, the AtNug2 knockdown mutant constructed by the RNAi method showed defective growth on the medium containing cycloheximide. Expression pattern analysis revealed that the distribution of AtNug2 mainly in the meristematic region underlies its potential role in active plant growth. Finally, it is concluded that Nug2/Nog2p GTPase from mono- and didicotyledonous plants is linked to the pre-60S ribosome complex and actively processed 27S into 25S during the ribosomal large subunit maturation process, i.e. prior to export to the cytoplasm." @default.
• W1966551301 created "2016-06-24" @default.
• W1966551301 creator A5008582554 @default.
• W1966551301 creator A5011542702 @default.
• W1966551301 creator A5017184805 @default.
• W1966551301 creator A5032962877 @default.
• W1966551301 creator A5046277778 @default.
• W1966551301 creator A5058202339 @default.
• W1966551301 creator A5065226364 @default.
• W1966551301 creator A5076737114 @default.
• W1966551301 date "2011-03-01" @default.
• W1966551301 modified "2023-10-02" @default.
• W1966551301 title "Nuclear/Nucleolar GTPase 2 Proteins as a Subfamily of YlqF/YawG GTPases Function in Pre-60S Ribosomal Subunit Maturation of Mono- and Dicotyledonous Plants" @default.
• W1966551301 cites W1539503448 @default.
• W1966551301 cites W1554952163 @default.
• W1966551301 cites W1662648663 @default.
• W1966551301 cites W1970340990 @default.
• W1966551301 cites W1978373237 @default.
• W1966551301 cites W1999475411 @default.
• W1966551301 cites W2012987716 @default.
• W1966551301 cites W2022961142 @default.
• W1966551301 cites W2022984307 @default.
• W1966551301 cites W2024305595 @default.
• W1966551301 cites W2025060812 @default.
• W1966551301 cites W2026249269 @default.
• W1966551301 cites W2030965575 @default.
• W1966551301 cites W2037055277 @default.
• W1966551301 cites W2039461665 @default.
• W1966551301 cites W2040441524 @default.
• W1966551301 cites W2047024375 @default.
• W1966551301 cites W2048120284 @default.
• W1966551301 cites W2049587461 @default.
• W1966551301 cites W2054315165 @default.
• W1966551301 cites W2055634291 @default.
• W1966551301 cites W2056887448 @default.
• W1966551301 cites W2058904982 @default.
• W1966551301 cites W2063381787 @default.
• W1966551301 cites W2063908091 @default.
• W1966551301 cites W2065922541 @default.
• W1966551301 cites W2072271218 @default.
• W1966551301 cites W2077167882 @default.
• W1966551301 cites W2080720176 @default.
• W1966551301 cites W2087576558 @default.
• W1966551301 cites W2087926131 @default.
• W1966551301 cites W2093468914 @default.
• W1966551301 cites W2094830599 @default.
• W1966551301 cites W2094874234 @default.
• W1966551301 cites W2095426716 @default.
• W1966551301 cites W2099735662 @default.
• W1966551301 cites W2099996820 @default.
• W1966551301 cites W2107136603 @default.
• W1966551301 cites W2110552809 @default.
• W1966551301 cites W2114460420 @default.
• W1966551301 cites W2116360991 @default.
• W1966551301 cites W2117926042 @default.
• W1966551301 cites W2118502991 @default.
• W1966551301 cites W2119515698 @default.
• W1966551301 cites W2124693268 @default.
• W1966551301 cites W2126193803 @default.
• W1966551301 cites W2126219914 @default.
• W1966551301 cites W2130031459 @default.
• W1966551301 cites W2148417715 @default.
• W1966551301 cites W2152038133 @default.
• W1966551301 cites W2153332247 @default.
• W1966551301 cites W2160552816 @default.
• W1966551301 cites W2165093821 @default.
• W1966551301 cites W4239681659 @default.
• W1966551301 doi "https://doi.org/10.1074/jbc.m110.200816" @default.
• W1966551301 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3048744" @default.
• W1966551301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21205822" @default.
• W1966551301 hasPublicationYear "2011" @default.
• W1966551301 type Work @default.
• W1966551301 sameAs 1966551301 @default.
• W1966551301 citedByCount "20" @default.
• W1966551301 countsByYear W19665513012012 @default.
• W1966551301 countsByYear W19665513012013 @default.
• W1966551301 countsByYear W19665513012014 @default.
• W1966551301 countsByYear W19665513012015 @default.
• W1966551301 countsByYear W19665513012017 @default.
• W1966551301 countsByYear W19665513012019 @default.
• W1966551301 countsByYear W19665513012020 @default.
• W1966551301 countsByYear W19665513012021 @default.
• W1966551301 countsByYear W19665513012022 @default.
• W1966551301 countsByYear W19665513012023 @default.
• W1966551301 crossrefType "journal-article" @default.
• W1966551301 hasAuthorship W1966551301A5008582554 @default.
• W1966551301 hasAuthorship W1966551301A5011542702 @default.
• W1966551301 hasAuthorship W1966551301A5017184805 @default.
• W1966551301 hasAuthorship W1966551301A5032962877 @default.
• W1966551301 hasAuthorship W1966551301A5046277778 @default.
• W1966551301 hasAuthorship W1966551301A5058202339 @default.
• W1966551301 hasAuthorship W1966551301A5065226364 @default.
• W1966551301 hasAuthorship W1966551301A5076737114 @default.
• W1966551301 hasBestOaLocation W19665513011 @default.
• W1966551301 hasConcept C104317684 @default.
• W1966551301 hasConcept C112755997 @default.
• W1966551301 hasConcept C129194359 @default.
• W1966551301 hasConcept C143065580 @default.

• next