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- W1966557248 abstract "A method is described for the isolation of lysosomes from rat liver in quantities greater than 60 mg. The method is based on the use of differential centrifugation and density- gradient centrifugation techniques. Biochemical studies show that lysosomes prepared in this manner are free from mitochondrial succinoxidase-system activity, not significantly contaminated with uricase particles but contain approx. 6–10% microsomal contamination. Studies were made of the stability of the lysosomes and of the release of lysosomal enzymes. In 0.7 M sucrose at 0–4°, 90–95% of the lysosomal enzymes remain latent for over 48 h indicating the stability of lysosomes under these conditions. The swelling or bursting of lysosomes as indicated by changes in light- scattering properties occurs at reduced osmotic pressure. This phenomenon is accompanied by a release of lysosomal enzymes. Studies of the release of the lysosomal enzymes suggest that these enzymes are not bound to the membrane as a lipoprotein complex. The composition of lysosomes is discussed." @default.
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- W1966557248 date "1964-04-01" @default.
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- W1966557248 title "Isolation of rat-liver lysosomes and their general properties" @default.
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- W1966557248 doi "https://doi.org/10.1016/0926-6569(64)90169-5" @default.
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