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- W1966562252 abstract "Transforming growth factor-beta (TGF-β) has been identified as an important component of wound healing. Recent developments in molecular therapy offer exciting prospects for the modulation of wound healing, specifically those targeting TGF-β. The purpose of this study was to analyze the effect of TGF-β targeting on the expression of angiogenic vascular endothelial growth factor (VEGF), a key regulator of angiogenesis, and in vitro angiogenic activity. Expression of angiogenic VEGF in tissue samples from chronic dermal wounds was investigated by immunohistochemistry. The effect of TGF-β targeting using antisense oligonucleotides on the expression of VEGF was analyzed by ELISA and RT-PCR in cultured human keratinocytes. Human endothelial cells (EC) were grown in conditioned medium produced from the treated keratinocytes. EC migration was measured using a modified Boyden chamber, EC tube formation was analyzed under the light microscope. Immunohistochemical investigation demonstrated a decreased expression of VEGF protein in tissue samples from chronic dermal wounds compared to normal human skin. Antisense TGF-β oligonucleotide treatment upregulated VEGF secretion in vitro. Addition of conditioned medium from TGF-β antisense-treated keratinocytes resulted in an increase of endothelial cell migration and tube formation. Our results demonstrate that TGF-β antisense oligonucleotide technology may be a potential therapeutic option for stimulation of angiogenesis in chronic wounds." @default.
- W1966562252 created "2016-06-24" @default.
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- W1966562252 date "2007-01-01" @default.
- W1966562252 modified "2023-09-23" @default.
- W1966562252 title "TGF-β Antisense Therapy Increases Angiogenic Potential in Human Keratinocytes In Vitro∗" @default.
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- W1966562252 doi "https://doi.org/10.1016/j.arcmed.2006.04.010" @default.
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