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- W19665712 abstract "In vitro dry matter digestibility is one of the most important characteristics of forages. Lignification of cell walls during plant development has been identified as the major factor limiting forage digestibility and concomitantly animal productivity. cDNA sequences encoding key lignin biosynthetic enzymes, cinnamyl alcohol dehydrogenase (CAD) and caffeic acid O-methyltransferase (COMT), were cloned from tall fescue (Festuca arundinacea Schreb.). Transgenic tall fescue plants carrying either sense or antisense CAD and COMT gene constructs were obtained by microprojectile bombardment of single genotype-derived embryogenic suspension cells. Severely reduced mRNA levels and significantly decreased enzymatic activities were found in four transgenic lines. These transgenic tall fescue plants had reduced lignin content, altered lignin composition and increased in vitro dry matter digestibility. No significant changes in cellulose, hemicellulose, neutral sugar composition, p-coumaric acid and ferulic acid levels were observed in the transgenic plants. This is the first time that a widely-grown forage grass species with modified lignin and improved digestibility was obtained by genetic engineering. Progresses in lignin modification of other plant species were also discussed.Keywordsforage grassdigestibilitylignin biosynthesistransgenic planttall fescue" @default.
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- W19665712 date "2006-02-22" @default.
- W19665712 modified "2023-10-02" @default.
- W19665712 title "Improving Forage Quality of Tall Fescue (Festuca arundinacea) by Genetic Manipulation of Lignin Biosynthesis" @default.
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- W19665712 doi "https://doi.org/10.1007/1-4020-2591-2_16" @default.
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