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- W1966612536 abstract "A novel and sensitive noncompetitive enzyme immunoassay for kassinin is described. Kassinin was biotinylated using sulfosuccinimidyl-6-(biotinamido)hexanoate. The biotinylated kassinin was trapped on anti-kassinin IgG-coated polystyrene balls and, after washing to eliminate other biotinylated substances, was eluted with HCl. The biotinylated kassinin eluted was reacted with anti-kassinin Fab'-peroxidase conjugate and trapped onto streptavidin-coated polystyrene balls. Peroxidase activity bound to the polystyrene balls was assayed by fluorimetry. The detection limit of kassinin was 0.13 pg (0.1 fmol)/tube or 0.065 microgram/l of rat plasma, which was 750-fold or 15-fold lower than that for competitive radioimmunoassay." @default.
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- W1966612536 date "1989-05-01" @default.
- W1966612536 modified "2023-09-27" @default.
- W1966612536 title "Novel and sensitive noncompetitive enzyme immunoassay for kassinin in rat plasma" @default.
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- W1966612536 doi "https://doi.org/10.1007/bf01952033" @default.
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