FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966648506> ?p ?o ?g. }

• W1966648506 endingPage "1125" @default.
• W1966648506 startingPage "1119" @default.
• W1966648506 abstract "Immobilised enzymes are widely used in industry, but the reasons for loss of activity of such biocatalysts are usually not known. We have used circular dichroism (CD) to investigate the structure of one such system, i.e., subtilisin Carlsberg (SC) immobilised on silica gel particles (60 μm). A number of technical problems have to be overcome in order to obtain appropriate data from which conclusions can be drawn. A rotating cell holder has been developed to avoid sedimentation of the silica particles during the collection of spectra. By moving the cell holder as close as possible to the detector window, the effects of differential scattering can be minimised. However, the effects of absorption flattening limit the extent to which reliable quantitative information on secondary structure content can be obtained from far UV CD studies. We have used an empirical approach based on absorbance units derived from the high-tension voltage to correct for absorption flattening effects. After applying the correction there was satisfactory agreement with the solution spectra. Comparison of the fresh and used (inactive) SC-silica gel spectra in organic media reveals substantial change in the secondary structure. Additional evidence for loss of native conformation is provided by the significant decrease in the near UV CD spectrum. These results for the first time clearly demonstrate the origin of enzyme instability in the immobilised state." @default.
• W1966648506 created "2016-06-24" @default.
• W1966648506 creator A5027276926 @default.
• W1966648506 creator A5035856070 @default.
• W1966648506 creator A5037846643 @default.
• W1966648506 creator A5050431593 @default.
• W1966648506 creator A5072188170 @default.
• W1966648506 creator A5076901096 @default.
• W1966648506 date "2006-06-01" @default.
• W1966648506 modified "2023-10-17" @default.
• W1966648506 title "Circular dichroism studies of subtilisin Carlsberg immobilised on micron sized silica particles" @default.
• W1966648506 cites W1976132571 @default.
• W1966648506 cites W1981143373 @default.
• W1966648506 cites W1983176521 @default.
• W1966648506 cites W2004709088 @default.
• W1966648506 cites W2006263216 @default.
• W1966648506 cites W2011642884 @default.
• W1966648506 cites W2014743473 @default.
• W1966648506 cites W2016865466 @default.
• W1966648506 cites W2023440735 @default.
• W1966648506 cites W2041374005 @default.
• W1966648506 cites W2060822393 @default.
• W1966648506 cites W2064262345 @default.
• W1966648506 cites W2072956040 @default.
• W1966648506 cites W2079564988 @default.
• W1966648506 cites W2085238030 @default.
• W1966648506 cites W2087135981 @default.
• W1966648506 cites W2092168842 @default.
• W1966648506 cites W2094331619 @default.
• W1966648506 cites W2104571730 @default.
• W1966648506 cites W2106939834 @default.
• W1966648506 cites W2107677733 @default.
• W1966648506 cites W2131977260 @default.
• W1966648506 cites W2134057677 @default.
• W1966648506 cites W2140260269 @default.
• W1966648506 cites W2147367306 @default.
• W1966648506 cites W2154844792 @default.
• W1966648506 cites W2157821027 @default.
• W1966648506 cites W2164940717 @default.
• W1966648506 doi "https://doi.org/10.1016/j.bbapap.2006.03.016" @default.
• W1966648506 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16716777" @default.
• W1966648506 hasPublicationYear "2006" @default.
• W1966648506 type Work @default.
• W1966648506 sameAs 1966648506 @default.
• W1966648506 citedByCount "36" @default.
• W1966648506 countsByYear W19666485062012 @default.
• W1966648506 countsByYear W19666485062013 @default.
• W1966648506 countsByYear W19666485062015 @default.
• W1966648506 countsByYear W19666485062019 @default.
• W1966648506 countsByYear W19666485062021 @default.
• W1966648506 countsByYear W19666485062022 @default.
• W1966648506 crossrefType "journal-article" @default.
• W1966648506 hasAuthorship W1966648506A5027276926 @default.
• W1966648506 hasAuthorship W1966648506A5035856070 @default.
• W1966648506 hasAuthorship W1966648506A5037846643 @default.
• W1966648506 hasAuthorship W1966648506A5050431593 @default.
• W1966648506 hasAuthorship W1966648506A5072188170 @default.
• W1966648506 hasAuthorship W1966648506A5076901096 @default.
• W1966648506 hasConcept C113196181 @default.
• W1966648506 hasConcept C120665830 @default.
• W1966648506 hasConcept C121332964 @default.
• W1966648506 hasConcept C125287762 @default.
• W1966648506 hasConcept C1276947 @default.
• W1966648506 hasConcept C133571119 @default.
• W1966648506 hasConcept C159985019 @default.
• W1966648506 hasConcept C178790620 @default.
• W1966648506 hasConcept C181199279 @default.
• W1966648506 hasConcept C185592680 @default.
• W1966648506 hasConcept C191486275 @default.
• W1966648506 hasConcept C192562407 @default.
• W1966648506 hasConcept C19444555 @default.
• W1966648506 hasConcept C2779811187 @default.
• W1966648506 hasConcept C43617362 @default.
• W1966648506 hasConcept C4839761 @default.
• W1966648506 hasConcept C8010536 @default.
• W1966648506 hasConcept C98015330 @default.
• W1966648506 hasConceptScore W1966648506C113196181 @default.
• W1966648506 hasConceptScore W1966648506C120665830 @default.
• W1966648506 hasConceptScore W1966648506C121332964 @default.
• W1966648506 hasConceptScore W1966648506C125287762 @default.
• W1966648506 hasConceptScore W1966648506C1276947 @default.
• W1966648506 hasConceptScore W1966648506C133571119 @default.
• W1966648506 hasConceptScore W1966648506C159985019 @default.
• W1966648506 hasConceptScore W1966648506C178790620 @default.
• W1966648506 hasConceptScore W1966648506C181199279 @default.
• W1966648506 hasConceptScore W1966648506C185592680 @default.
• W1966648506 hasConceptScore W1966648506C191486275 @default.
• W1966648506 hasConceptScore W1966648506C192562407 @default.
• W1966648506 hasConceptScore W1966648506C19444555 @default.
• W1966648506 hasConceptScore W1966648506C2779811187 @default.
• W1966648506 hasConceptScore W1966648506C43617362 @default.
• W1966648506 hasConceptScore W1966648506C4839761 @default.
• W1966648506 hasConceptScore W1966648506C8010536 @default.
• W1966648506 hasConceptScore W1966648506C98015330 @default.
• W1966648506 hasIssue "6" @default.
• W1966648506 hasLocation W19666485061 @default.
• W1966648506 hasLocation W19666485062 @default.
• W1966648506 hasOpenAccess W1966648506 @default.

• next