Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966650078> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1966650078 endingPage "779" @default.
- W1966650078 startingPage "771" @default.
- W1966650078 abstract "Carboxy-terminal amino acids of NADP-dependent malate dehydrogenase (EC 1.1.1.82) from pea chloroplasts were removed by treatment with carboxypeptidase Y. This results in the activation of the inactive oxidized enzyme, while activation by light in vivo is thought to occur via reduction of an intrasubunit disulfide bridge. After proteolytic activation the oxidized enzyme had a specific activity of 100 U/mg protein, which is 50% of the maximal activity of the control enzyme in the reduced state. When the truncated enzyme was reduced with dithiothreitol (DTT), the specific activity was further increased to 1200 U/mg. While the native enzyme is composed of four identical subunits of 38,900 Da, the truncated malate dehydrogenase forms dimers composed of two subunits of 38,000 Da. No further change of molecular mass or activity was noticed subsequent to prolonged incubation of native NADP-malate dehydrogenase with carboxypeptidase Y for several days. When the enzyme is denatured by 2 M guanidine-HCl, the proteolytic activation proceeds more rapidly, but only transiently. The truncated enzyme is less accessible to activation by reduced thioredoxin, but the stimulation of activity by DTT alone is more rapid than that of the native enzyme. These results indicate that only a small carboxy-terminal peptide of native NADP-malate dehydrogenase from pea chloroplasts is accessible to proteolytic degradation and that this peptide is involved in the regulation of activity, tetramer formation, and thioredoxin binding. While the pH optimum for catalytic activity of the intact reduced enzyme is at pH 8.0-8.5, it is shifted to more acidic values upon proteolysis of NADP-malate dehydrogenase. At pH values below 8 the reduced truncated enzyme exhibits substrate inhibition by oxaloacetate." @default.
- W1966650078 created "2016-06-24" @default.
- W1966650078 creator A5033315561 @default.
- W1966650078 creator A5091461544 @default.
- W1966650078 date "1988-02-01" @default.
- W1966650078 modified "2023-10-16" @default.
- W1966650078 title "Limited proteolysis of inactive tetrameric chloroplast NADP-Malate dehydrogenase produces active dimers" @default.
- W1966650078 cites W1537362115 @default.
- W1966650078 cites W1563334886 @default.
- W1966650078 cites W1642157127 @default.
- W1966650078 cites W186445262 @default.
- W1966650078 cites W1966393352 @default.
- W1966650078 cites W1973528267 @default.
- W1966650078 cites W1990052753 @default.
- W1966650078 cites W2030823625 @default.
- W1966650078 cites W2033845198 @default.
- W1966650078 cites W2042184304 @default.
- W1966650078 cites W2044712249 @default.
- W1966650078 cites W2044841188 @default.
- W1966650078 cites W2049986828 @default.
- W1966650078 cites W2052533514 @default.
- W1966650078 cites W2059402446 @default.
- W1966650078 cites W2062600212 @default.
- W1966650078 cites W2064804975 @default.
- W1966650078 cites W2071988883 @default.
- W1966650078 cites W2072059333 @default.
- W1966650078 cites W2074532552 @default.
- W1966650078 cites W2084439033 @default.
- W1966650078 cites W2092383329 @default.
- W1966650078 cites W2100837269 @default.
- W1966650078 cites W2320003067 @default.
- W1966650078 cites W2321798561 @default.
- W1966650078 doi "https://doi.org/10.1016/0003-9861(88)90507-3" @default.
- W1966650078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3341764" @default.
- W1966650078 hasPublicationYear "1988" @default.
- W1966650078 type Work @default.
- W1966650078 sameAs 1966650078 @default.
- W1966650078 citedByCount "35" @default.
- W1966650078 countsByYear W19666500782013 @default.
- W1966650078 countsByYear W19666500782015 @default.
- W1966650078 countsByYear W19666500782018 @default.
- W1966650078 countsByYear W19666500782020 @default.
- W1966650078 countsByYear W19666500782021 @default.
- W1966650078 crossrefType "journal-article" @default.
- W1966650078 hasAuthorship W1966650078A5033315561 @default.
- W1966650078 hasAuthorship W1966650078A5091461544 @default.
- W1966650078 hasConcept C104317684 @default.
- W1966650078 hasConcept C108477463 @default.
- W1966650078 hasConcept C125209161 @default.
- W1966650078 hasConcept C132901563 @default.
- W1966650078 hasConcept C181199279 @default.
- W1966650078 hasConcept C185592680 @default.
- W1966650078 hasConcept C2776317432 @default.
- W1966650078 hasConcept C2777824588 @default.
- W1966650078 hasConcept C2778886173 @default.
- W1966650078 hasConcept C2781307694 @default.
- W1966650078 hasConcept C28212737 @default.
- W1966650078 hasConcept C3623737 @default.
- W1966650078 hasConcept C55493867 @default.
- W1966650078 hasConcept C69305403 @default.
- W1966650078 hasConcept C86803240 @default.
- W1966650078 hasConceptScore W1966650078C104317684 @default.
- W1966650078 hasConceptScore W1966650078C108477463 @default.
- W1966650078 hasConceptScore W1966650078C125209161 @default.
- W1966650078 hasConceptScore W1966650078C132901563 @default.
- W1966650078 hasConceptScore W1966650078C181199279 @default.
- W1966650078 hasConceptScore W1966650078C185592680 @default.
- W1966650078 hasConceptScore W1966650078C2776317432 @default.
- W1966650078 hasConceptScore W1966650078C2777824588 @default.
- W1966650078 hasConceptScore W1966650078C2778886173 @default.
- W1966650078 hasConceptScore W1966650078C2781307694 @default.
- W1966650078 hasConceptScore W1966650078C28212737 @default.
- W1966650078 hasConceptScore W1966650078C3623737 @default.
- W1966650078 hasConceptScore W1966650078C55493867 @default.
- W1966650078 hasConceptScore W1966650078C69305403 @default.
- W1966650078 hasConceptScore W1966650078C86803240 @default.
- W1966650078 hasIssue "2" @default.
- W1966650078 hasLocation W19666500781 @default.
- W1966650078 hasLocation W19666500782 @default.
- W1966650078 hasOpenAccess W1966650078 @default.
- W1966650078 hasPrimaryLocation W19666500781 @default.
- W1966650078 hasRelatedWork W1966650078 @default.
- W1966650078 hasRelatedWork W1966908421 @default.
- W1966650078 hasRelatedWork W1978189929 @default.
- W1966650078 hasRelatedWork W1988346978 @default.
- W1966650078 hasRelatedWork W2005079416 @default.
- W1966650078 hasRelatedWork W2010838233 @default.
- W1966650078 hasRelatedWork W2032820794 @default.
- W1966650078 hasRelatedWork W2041117277 @default.
- W1966650078 hasRelatedWork W2046019655 @default.
- W1966650078 hasRelatedWork W2314877183 @default.
- W1966650078 hasVolume "260" @default.
- W1966650078 isParatext "false" @default.
- W1966650078 isRetracted "false" @default.
- W1966650078 magId "1966650078" @default.
- W1966650078 workType "article" @default.