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- W1966678865 abstract "Summary Former investigations comparing the anti-tick-borne encephalitis virus (TBEV) seroprevalence in risk-professions versus the general population in both the endemic region of southwestern Germany and the neighbouring North-East of France (Alsatia) revealed similar rates of seropositive individuals in both geographic areas. This was in discord with the known number of TBE patients: whereas up to 250 cases per year were observed in southwestern Germany, only about 30 patients were reported from the adjacent French region over the past 30 years. In order to exclude unspecific results in the ELISA testing, a Western blot method was established using highly purified TBE virus ( strain K23) as antigen. The method was evaluated on the one hand by means of known anti-TBEV-negative sera from healthy individuals and patients suffering from rheumatic disorders and, on the other, by using a seroconversion panel as well as sera from patients with known, clinically confirmed TBEV infection. After test evaluation, ELISA-positive and ELISA-borderline sera of 248 German and 94 French individuals were retested by means of the Western blot method. Considering the ELISA-positive sera, 63.9% of the German (50.0% if only non-vaccinated individuals were considered) but only 10.5% of the French samples could be confirmed by Western blot. This difference in confirmation is highly significant (p" @default.
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- W1966678865 date "1999-12-01" @default.
- W1966678865 modified "2023-09-27" @default.
- W1966678865 title "Detection of antibodies against TBE virus by means of Western blot: Confirmation of a high level of false positive ELISA results in a population with low TBE incidence" @default.
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- W1966678865 doi "https://doi.org/10.1016/s0934-8840(99)80019-x" @default.
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