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- W1966679920 abstract "Defining the role of cholecystokinin (CCK) in gut physiology and disease has proved difficult because of problems with development of radioimmunoassays and because CCK exists in several different molecular forms which have different biological actions. In order to measure small (8 amino acid residues, CCK 8) and large (33 and 39 residues) forms of CCK in plasma we have developed high pressure liquid chromatographic (HPLC) fractionation of plasma prior to radioimmunoassay. Fasting plasma CCK 8 and CCK 3339 levels were usually undetectable (< 3 and < 6 pmol/1, respectively). After a liquid fat meal both CCK 8 and CCK 3339 levels were significantly elevated at 5 min (11.3±3.3 and 11.6±2.6 pmol/1, respectively). Peak CCK 8 levels occurred at 30 min (15.0±4.4 pmol/1) while peak CCK 3339 levels occurred at 120 min (16.7±4.9 pmol/1. Total CCK levels showed a biphasic response to the meal. These CCK 8 and CCK 3339 responses to oral fat are consistent with a role for these hormones in the regulation of gallbladder emptying and pancreatic secretion." @default.
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- W1966679920 date "1982-10-01" @default.
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- W1966679920 title "Large and small forms of cholecystokinin in human plasma: measurement using high pressure liquid chromatography and radioimmunoassay" @default.
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- W1966679920 doi "https://doi.org/10.1016/0167-0115(82)90118-5" @default.
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