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- W1966740873 abstract "Gradient elution reversed-phase high-performance liquid chromatography (RP-HPLC) was used for the determination of compounds occurring during the production of biodiesel from rapeseed oil. Individual triacylglycerols (TGs), diacylglycerols, monoacylglycerols and methyl esters of oleic, linoleic and linolenic acids and free fatty acids were separated in 25 min using a combined linear gradient with aqueous–organic and non-aqueous mobile phase steps: 70% acetonitrile+30% water in 0 min, 100% acetonitrile in 10 min, 50% acetonitrile+50% 2-propanol–hexane (5:4, v/v) in 20 min and 5 min final hold-up. Another method with a non-aqueous linear mobile phase gradient [from 100% methanol to 50% methanol+50% 2-propanol–hexane (5:4, v/v) in 15 min] was used for fast monitoring of conversion of rapeseed oil triacylglycerols to fatty acid methyl esters and for quantitation of residual TGs in the final biodiesel product. Sensitivity and linearity of various detection modes (UV detection at 205 nm, evaporative light scattering detection and mass spectrometric detection) were compared. The individual sample compounds were identified using coupled HPLC–atmospheric pressure chemical ionization mass spectrometry in the positive-ion mode." @default.
- W1966740873 created "2016-06-24" @default.
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- W1966740873 date "1999-10-01" @default.
- W1966740873 modified "2023-10-18" @default.
- W1966740873 title "Analytical monitoring of the production of biodiesel by high-performance liquid chromatography with various detection methods" @default.
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- W1966740873 doi "https://doi.org/10.1016/s0021-9673(99)00790-6" @default.
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