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- W1966756632 abstract "A comprehensive understanding of the cell biology of adult neural stem cells (aNSCs) requires direct observation of aNSC division and lineage progression in the absence of niche-dependent signals. Here we describe a culture preparation of the adult mouse subependymal zone (SEZ), which allows for continuous single-cell tracking of aNSC behavior. The protocol involves the isolation (∼3 h) and culture of cells from the adult SEZ at low density in the absence of mitogenic growth factors in chemically defined medium and subsequent live imaging using time-lapse video microscopy (5–7 d); these steps are followed by postimaging immunocytochemistry to identify progeny (∼7 h). This protocol enables the observation of the progression from slow-dividing aNSCs of radial/astroglial identity up to the neuroblast stage, involving asymmetric and symmetric cell divisions of distinct fast-dividing precursors. This culture provides an experimental system for studying instructive or permissive effects of signal molecules on aNSC modes of cell division and lineage progression." @default.
- W1966756632 created "2016-06-24" @default.
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- W1966756632 date "2011-11-03" @default.
- W1966756632 modified "2023-10-16" @default.
- W1966756632 title "Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level" @default.
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- W1966756632 doi "https://doi.org/10.1038/nprot.2011.404" @default.
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