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- W1966792303 abstract "Animal embryo development exhibits a complex ensemble of cell movements that are tightly regulated by developmental gene expression. It was proposed recently that mechanical factors may also play an important role during development. Investigating these dynamical processes is technically challenging and requires novel <i>in vivo</i> investigation methods. We show that multiphoton microscopy can be used for both perturbing and analyzing morphogenetic movements in vivo. (i) nonlinear microscopy is well adapted for the sustained imaging of early Drosophila embryos despite their highly scattering nature; (ii) femtosecond pulse-induced ablation can be used to process specific tissues <i>in vivo.</i> Combining this approach with multimodal microscopy (two-photon-excited fluorescence (2PEF) and third-harmonic generation (THG)), we report the successful quantitative modulation of morphogenetic movements <i>in vivo</i>. Our data provides insight to the issue of morphogenesis regulation." @default.
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- W1966792303 date "2005-03-30" @default.
- W1966792303 modified "2023-10-17" @default.
- W1966792303 title "In vivo analysis of Drosophila embryo developmental dynamics by femtosecond pulse-induced ablation and multimodal nonlinear microscopy" @default.
- W1966792303 doi "https://doi.org/10.1117/12.589425" @default.
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