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- W1966797079 abstract "Abstract The binding of 3 H-diisopropyl fluorophosphate (DFP) to cholinesterase sites in the rat corpus striatum was studied by means of electron microscopic autoradiography. Small blocks of fixed tissue were incubated in the sequence nonradioactive DFP: Pyridine-2-aldoxime methiodide (2-PAM): 3 H-DFP. The 2-PAM was used as a selective regenerating agent for cholinesterase sites prior to incubation with 3 H-DFP to label the sites. Terminal boutons had 22% of the grains and the highest relative grain density (RGD = % grains/% area) of 2.20. Dendrites and dendritic spines had 38% of the grains and a relative grain density of 1.41. Other structures, such as cell somata, axons, glial processes and blood vessels, had the remainder of the grains and a relative grain density between 0.58 and 0.66. Striatal boutons were divided into four types: asymmetrical groups 1 and 2, and symmetrical groups 1 and 2. One type—asymmetrical group 1—was much more heavily labelled than the others, and was therefore presumed to be cholinergic. This type is densely packed with common round vesicles about 400–450 A in diameter and makes axospinous contacts." @default.
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- W1966797079 date "1974-12-01" @default.
- W1966797079 modified "2023-09-27" @default.
- W1966797079 title "Electron microscopic identification of cholinesterase-containing structures in the corpus striatum by diisopropyl fluorophosphate uptake" @default.
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- W1966797079 doi "https://doi.org/10.1016/0014-4886(74)90159-9" @default.
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