FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966841279> ?p ?o ?g. }

• W1966841279 endingPage "366" @default.
• W1966841279 startingPage "363" @default.
• W1966841279 abstract "In Gram-positive bacteria, T-box riboswitches regulate the expression of aminoacyl-tRNA synthetases and other proteins in response to fluctuating transfer RNA aminoacylation levels under various nutritional states. T-boxes reside in the 5'-untranslated regions of the messenger RNAs they regulate, and consist of two conserved domains. Stem I contains the specifier trinucleotide that base pairs with the anticodon of cognate tRNA. 3' to stem I is the antiterminator domain, which base pairs with the tRNA acceptor end and evaluates its aminoacylation state. Despite high phylogenetic conservation and widespread occurrence in pathogens, the structural basis of tRNA recognition by this riboswitch remains ill defined. Here we demonstrate that the ~100-nucleotide T-box stem I is necessary and sufficient for specific, high-affinity (dissociation constant (Kd) ~150 nM) tRNA binding, and report the structure of Oceanobacillus iheyensis glyQ stem I in complex with its cognate tRNA at 3.2 Å resolution. Stem I recognizes the overall architecture of tRNA in addition to its anticodon, something accomplished by large ribonucleoproteins such as the ribosome, or proteins such as aminoacyl-tRNA synthetases, but is unprecedented for a compact mRNA domain. The C-shaped stem I cradles the L-shaped tRNA, forming an extended (1,604 Å(2)) intermolecular interface. In addition to the specifier-anticodon interaction, two interdigitated T-loops near the apex of stem I stack on the tRNA elbow in a manner analogous to those of the J11/12-J12/11 motif of RNase P and the L1 stalk of the ribosomal E-site. Because these ribonucleoproteins and T-boxes are unrelated, this strategy to recognize a universal tRNA feature probably evolved convergently. Mutually induced fit of stem I and the tRNA exploiting the intrinsic flexibility of tRNA and its conserved post-transcriptional modifications results in high shape complementarity, which in addition to providing specificity and affinity, globally organizes the T-box to orchestrate tRNA-dependent transcription regulation." @default.
• W1966841279 created "2016-06-24" @default.
• W1966841279 creator A5047073489 @default.
• W1966841279 creator A5084331390 @default.
• W1966841279 date "2013-07-28" @default.
• W1966841279 modified "2023-10-02" @default.
• W1966841279 title "Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA" @default.
• W1966841279 cites W1484664209 @default.
• W1966841279 cites W1539796472 @default.
• W1966841279 cites W1973733443 @default.
• W1966841279 cites W1975317507 @default.
• W1966841279 cites W1980461282 @default.
• W1966841279 cites W1981712446 @default.
• W1966841279 cites W1986221874 @default.
• W1966841279 cites W1986414204 @default.
• W1966841279 cites W1987177052 @default.
• W1966841279 cites W2003026778 @default.
• W1966841279 cites W2009746802 @default.
• W1966841279 cites W2022754910 @default.
• W1966841279 cites W2023209608 @default.
• W1966841279 cites W2026004038 @default.
• W1966841279 cites W2026817175 @default.
• W1966841279 cites W2028556267 @default.
• W1966841279 cites W2034074153 @default.
• W1966841279 cites W2063894914 @default.
• W1966841279 cites W2070411828 @default.
• W1966841279 cites W2075346950 @default.
• W1966841279 cites W2079204867 @default.
• W1966841279 cites W2081981294 @default.
• W1966841279 cites W2082592915 @default.
• W1966841279 cites W2091218156 @default.
• W1966841279 cites W2093041571 @default.
• W1966841279 cites W2097493124 @default.
• W1966841279 cites W2101876170 @default.
• W1966841279 cites W2106478835 @default.
• W1966841279 cites W2107994759 @default.
• W1966841279 cites W2110118545 @default.
• W1966841279 cites W2114478647 @default.
• W1966841279 cites W2115855895 @default.
• W1966841279 cites W2124026197 @default.
• W1966841279 cites W2131350133 @default.
• W1966841279 cites W2132129663 @default.
• W1966841279 cites W2133324564 @default.
• W1966841279 cites W2137206793 @default.
• W1966841279 cites W2142640423 @default.
• W1966841279 cites W2142672816 @default.
• W1966841279 cites W2143694829 @default.
• W1966841279 cites W2145985654 @default.
• W1966841279 cites W2146912125 @default.
• W1966841279 cites W2149114938 @default.
• W1966841279 cites W2154197653 @default.
• W1966841279 cites W2155376412 @default.
• W1966841279 cites W2162528693 @default.
• W1966841279 cites W2163341755 @default.
• W1966841279 cites W2316970422 @default.
• W1966841279 cites W4210675630 @default.
• W1966841279 cites W4248872320 @default.
• W1966841279 doi "https://doi.org/10.1038/nature12440" @default.
• W1966841279 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3808885" @default.
• W1966841279 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23892783" @default.
• W1966841279 hasPublicationYear "2013" @default.
• W1966841279 type Work @default.
• W1966841279 sameAs 1966841279 @default.
• W1966841279 citedByCount "129" @default.
• W1966841279 countsByYear W19668412792013 @default.
• W1966841279 countsByYear W19668412792014 @default.
• W1966841279 countsByYear W19668412792015 @default.
• W1966841279 countsByYear W19668412792016 @default.
• W1966841279 countsByYear W19668412792017 @default.
• W1966841279 countsByYear W19668412792018 @default.
• W1966841279 countsByYear W19668412792019 @default.
• W1966841279 countsByYear W19668412792020 @default.
• W1966841279 countsByYear W19668412792021 @default.
• W1966841279 countsByYear W19668412792022 @default.
• W1966841279 countsByYear W19668412792023 @default.
• W1966841279 crossrefType "journal-article" @default.
• W1966841279 hasAuthorship W1966841279A5047073489 @default.
• W1966841279 hasAuthorship W1966841279A5084331390 @default.
• W1966841279 hasBestOaLocation W19668412792 @default.
• W1966841279 hasConcept C104317684 @default.
• W1966841279 hasConcept C105130999 @default.
• W1966841279 hasConcept C10879258 @default.
• W1966841279 hasConcept C148625055 @default.
• W1966841279 hasConcept C151238385 @default.
• W1966841279 hasConcept C153957851 @default.
• W1966841279 hasConcept C194993378 @default.
• W1966841279 hasConcept C203353237 @default.
• W1966841279 hasConcept C21312847 @default.
• W1966841279 hasConcept C2779315393 @default.
• W1966841279 hasConcept C54355233 @default.
• W1966841279 hasConcept C67705224 @default.
• W1966841279 hasConcept C86803240 @default.
• W1966841279 hasConcept C88478588 @default.
• W1966841279 hasConcept C95444343 @default.
• W1966841279 hasConceptScore W1966841279C104317684 @default.
• W1966841279 hasConceptScore W1966841279C105130999 @default.
• W1966841279 hasConceptScore W1966841279C10879258 @default.
• W1966841279 hasConceptScore W1966841279C148625055 @default.

• next