Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966849320> ?p ?o ?g. }
- W1966849320 endingPage "158" @default.
- W1966849320 startingPage "149" @default.
- W1966849320 abstract "The aim of this work was to investigate whether in vivo and in vitro pentoxifylline (PTX) sensitizes hematological tumor cells to adriamycin (ADM)-induced apoptosis, and to investigate the involvement of caspase cascades and phosphorylated forms of IkappaBalpha. Balb/c mice inoculated intraperitoneally with L5178-Y murine lymphoma cells were used for in vivo experiments and for survival studies. The U937 human monocytic cell line was used for in vitro experiments. Both cell lines were treated under similar experimental conditions with PTX and/or ADM to assess their effects on apoptosis. Apoptosis was evaluated by fluorescence microscopy with ethidium bromide and acridine orange staining and confirmed by electrophoretic DNA analysis. Caspase inhibitors Z-VAD-fmk, Z-DEVD-fmk, and Z-LEHD-fmk were used to investigate the involvement of caspase cascades. C-terminally and Ser32 phosphorylated forms of IkappaBalpha were evaluated in cytoplasmic extracts in the absence or presence of TNFalpha.In vivo, PTX (50 mg/kg) with ADM (5 mg/kg) increased the apoptotic index relative to PTX or ADM administered alone, time- and dose-dependently. DNA laddering appeared in lymphoma cells treated with PTX+ADM at 24 h, whereas neither untreated control, PTX-, nor ADM-treated cells showed DNA fragmentation. All (100%) tumor-bearing mice treated with PTX (25 mg/kg)+ADM (2.5 mg/kg) survived for 1 year, whereas the mortality rates of mice treated with either PTX or ADM alone at the same doses were similar to that of untreated tumor-bearing mice (28+/-3 days). Caspase inhibitors inhibited apoptosis more efficiently in PTX- or ADM-treated cultures than in PTX+ADM-treated cultures. Pretreatment with TNFalpha (10 ng/mL) increased apoptosis in PTX- or ADM-treated U937 cells. However, the apoptotic index of PTX+ADM-treated cultures was significantly reduced and the expression of C-terminally and Ser32 phosphorylated IkappaBalpha was reduced. PTX sensitizes hematological malignancies to ADR-induced apoptosis. An independent caspase pathway is involved in PTX+ADM-induced apoptosis. The phosphorylation status of IkappaBalpha is closely related via TNFalpha to the possible mechanisms of drug resistance." @default.
- W1966849320 created "2016-06-24" @default.
- W1966849320 creator A5016329536 @default.
- W1966849320 creator A5017598091 @default.
- W1966849320 creator A5020577954 @default.
- W1966849320 creator A5024019014 @default.
- W1966849320 creator A5031230291 @default.
- W1966849320 creator A5044293589 @default.
- W1966849320 creator A5056272703 @default.
- W1966849320 creator A5073967875 @default.
- W1966849320 creator A5085190195 @default.
- W1966849320 creator A5088205036 @default.
- W1966849320 date "2006-03-15" @default.
- W1966849320 modified "2023-10-15" @default.
- W1966849320 title "In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifyllineInvolvement of caspase cascades and IκBα phosphorylation" @default.
- W1966849320 cites W159669330 @default.
- W1966849320 cites W1830573358 @default.
- W1966849320 cites W1896161278 @default.
- W1966849320 cites W1899350146 @default.
- W1966849320 cites W1915074145 @default.
- W1966849320 cites W193483859 @default.
- W1966849320 cites W1963754854 @default.
- W1966849320 cites W1970945068 @default.
- W1966849320 cites W1976669514 @default.
- W1966849320 cites W1979461093 @default.
- W1966849320 cites W1982531566 @default.
- W1966849320 cites W1990688927 @default.
- W1966849320 cites W1995023187 @default.
- W1966849320 cites W1996813579 @default.
- W1966849320 cites W2000311561 @default.
- W1966849320 cites W2001707528 @default.
- W1966849320 cites W2004087697 @default.
- W1966849320 cites W2023214683 @default.
- W1966849320 cites W2024769251 @default.
- W1966849320 cites W2026023655 @default.
- W1966849320 cites W2031481616 @default.
- W1966849320 cites W2035280748 @default.
- W1966849320 cites W2042196731 @default.
- W1966849320 cites W2042376849 @default.
- W1966849320 cites W2046126741 @default.
- W1966849320 cites W2046893011 @default.
- W1966849320 cites W2054807590 @default.
- W1966849320 cites W2062208385 @default.
- W1966849320 cites W2065079479 @default.
- W1966849320 cites W2070853191 @default.
- W1966849320 cites W2072591613 @default.
- W1966849320 cites W2083302896 @default.
- W1966849320 cites W2084222157 @default.
- W1966849320 cites W2087056351 @default.
- W1966849320 cites W2091593426 @default.
- W1966849320 cites W2091666164 @default.
- W1966849320 cites W2095358270 @default.
- W1966849320 cites W2097792939 @default.
- W1966849320 cites W2098432885 @default.
- W1966849320 cites W2109134791 @default.
- W1966849320 cites W2112135370 @default.
- W1966849320 cites W2114279859 @default.
- W1966849320 cites W2120554134 @default.
- W1966849320 cites W2122233629 @default.
- W1966849320 cites W2124037996 @default.
- W1966849320 cites W2137506674 @default.
- W1966849320 cites W2151501496 @default.
- W1966849320 cites W2169970339 @default.
- W1966849320 cites W2172235735 @default.
- W1966849320 cites W22004154 @default.
- W1966849320 cites W2244276231 @default.
- W1966849320 cites W2326572877 @default.
- W1966849320 cites W2331722009 @default.
- W1966849320 cites W2335082157 @default.
- W1966849320 cites W2390238244 @default.
- W1966849320 cites W2406287373 @default.
- W1966849320 cites W2468781127 @default.
- W1966849320 cites W247885817 @default.
- W1966849320 cites W51229851 @default.
- W1966849320 cites W95988660 @default.
- W1966849320 doi "https://doi.org/10.1016/j.imlet.2005.10.019" @default.
- W1966849320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16388856" @default.
- W1966849320 hasPublicationYear "2006" @default.
- W1966849320 type Work @default.
- W1966849320 sameAs 1966849320 @default.
- W1966849320 citedByCount "30" @default.
- W1966849320 countsByYear W19668493202012 @default.
- W1966849320 countsByYear W19668493202013 @default.
- W1966849320 countsByYear W19668493202014 @default.
- W1966849320 countsByYear W19668493202015 @default.
- W1966849320 countsByYear W19668493202016 @default.
- W1966849320 countsByYear W19668493202017 @default.
- W1966849320 countsByYear W19668493202018 @default.
- W1966849320 countsByYear W19668493202019 @default.
- W1966849320 countsByYear W19668493202020 @default.
- W1966849320 countsByYear W19668493202021 @default.
- W1966849320 countsByYear W19668493202022 @default.
- W1966849320 countsByYear W19668493202023 @default.
- W1966849320 crossrefType "journal-article" @default.
- W1966849320 hasAuthorship W1966849320A5016329536 @default.
- W1966849320 hasAuthorship W1966849320A5017598091 @default.
- W1966849320 hasAuthorship W1966849320A5020577954 @default.
- W1966849320 hasAuthorship W1966849320A5024019014 @default.