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• W1966857565 abstract "Monoclonal anti-fluorescein isothiocyanate (FITC) antibody cross-reacts with 7-hydroxy coumarin derivatives conjugated to BSA. This property permitted the affinity purification of monoclonal anti-FITC antibodies from ascitic fluid using an-immunosorbent consisting of a 7-hydroxy coumarin derivative linked to Sepharose 4B. Ascitic fluid was applied to the immunosorbent column and, after washing, the bound antibody was eluted under extremely mild conditions using 3 M MgCl2. Antibody eluted in this manner was greater than 96% pure as assessed by SDS-PAGE. A polyclonal sheep anti-FITC antibody was also purified from serum on the same immunosorbent to greater than 94% purity. This simple and rapid method for the purification of anti-FITC antibodies will find applications in both immunodiagnostic procedures and in studies of hapten-antibody interactions. The affinity constant of the purified monoclonal anti-FITC antibody conjugated to horseradish peroxidase was assessed by ELISA and was found to be 1.5 x 19(9) M-1." @default.
• W1966857565 created "2016-06-24" @default.
• W1966857565 creator A5054206253 @default.
• W1966857565 creator A5080203252 @default.
• W1966857565 date "1990-10-01" @default.
• W1966857565 modified "2023-09-23" @default.
• W1966857565 title "A single-step method for the purification of anti-FITC antibodies by use of a coumarin immunosorbent" @default.
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• W1966857565 doi "https://doi.org/10.1016/0022-1759(90)90327-r" @default.
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• W1966857565 hasPublicationYear "1990" @default.
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