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- W1966886551 abstract "5–9 different molecular species of phosphatidylinositol (PI) were separated, combining HPLC and GLC analyses, from microsomes extracted from potato tuber, pea leaf and germinated soya beans. In microsomes from the three plant tissues, 16:0/18:2-PI was the most abundant molecular species. The second most abundant species was 16:0/18:3-PI in pea leaf and potato tuber, whereas 18:0/18:2-PI was more abundant than 16:0/18:3-PI in soya bean microsomes. Labelling PI molecular species from [14C]myo-inositol, in the presence of EDTA to avoid any exchange reaction, showed that only two molecular species (16:0/18:2-PI and 16:0/18:3-PI) were prominently produced by PI synthases from the three tissues. The molecular species 18:0/18:2-PI and 18:2/18:3-PI were slightly labelled (less than 10% of total PI radioactivity) in soya bean and pea leaf, respectively. A good correspondence could be observed between the quantitative distributions of PI molecular species and the relative labellings of the same species in the three tissues. Those results suggest that de novo synthesis of PI molecular species is not followed by subsequent alterations (acyl exchanges or desaturations) of these molecules." @default.
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- W1966886551 date "1989-09-01" @default.
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- W1966886551 title "Molecular species synthesized by phosphatidylinositol synthases from potato tuber, pea leaf and soya bean" @default.
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- W1966886551 doi "https://doi.org/10.1016/0005-2760(89)90030-1" @default.
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