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- W1966886688 abstract "Abstract The resolution and specificity of 1 H NMR in studies of yeast cellular metabolism were increased by feeding a 13 C-labeled substrate and observing 1 H difference spectra in the presence and absence of 13 C decoupling fields. [2- 13 C]Acetate was utilized as a respiratory substrate in an aerobic suspension of Saccharomyces cerevisiae . The broad cellular background proton resonances are removed by the technique, leaving only signals from the protons of the substrate, or its metabolites, that are coupled to 13 C. Spectra of the yeast suspension after acetate feeding show the disappearance of label from the acetate pool and the subsequent appearance of 13 C in glutamate C 3 and C 4 and in aspartate C 3 . These results are in accord with the known fluxes of metabolites. Selective single-frequency 13 C decoupling was used to provide assignments for the difference signals. The limitations on single-frequency decoupling coming from finite decoupling fields are investigated. The technique shows a potential for application in a wide variety of systems where the resolution of the 13 C spectrum may be combined with the sensitivity for proton detection to observe metabolites that have been previously unobservable." @default.
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- W1966886688 date "1981-10-01" @default.
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- W1966886688 title "High-resolution proton NMR studies of intracellular metabolites in yeast using 13C decoupling" @default.
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- W1966886688 doi "https://doi.org/10.1016/0022-2364(81)90108-6" @default.
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