Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966940007> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1966940007 endingPage "49" @default.
- W1966940007 startingPage "49e" @default.
- W1966940007 abstract "A new method using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for the direct analysis of the mass-silent post-transcriptionally modified nucleoside pseudouridine in nucleic acids has been developed. This method utilizes 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide to derivatize pseudouridine residues. After chemical derivatization all pseudouridine residues will contain a 252 Da 'mass tag' that allows the presence of pseudouridine to be identified using mass spectrometry. Pseudouridine residues can be identified in intact nucleic acids by obtaining a mass spectrum of the nucleic acid before and after derivatization. The mass difference (in units of 252 Da) will denote the number of pseudouridine residues present. To determine the sequence location of pseudouridine, a combination of enzymatic hydrolysis and mass spectrometric steps are used. Here, MALDI analysis of RNase T1 digestion products before and after modification are used to narrow the sequence location of pseudouridine to specific T1 fragments in the gene sequence. Further mass spectrometric monitoring of exonuclease digestion products from isolated T1 fragments is then used for exact sequence placement. This approach to pseudouridine identification is demonstrated using Escherichia coli tRNAS: This new method allows for the direct determination of pseudouridine in nucleic acids, can be used to identify modified pseudouridine residues and can be used with general modification mapping approaches to completely characterize the post-transcriptional modifications present in RNAs." @default.
- W1966940007 created "2016-06-24" @default.
- W1966940007 creator A5072536402 @default.
- W1966940007 date "2001-05-15" @default.
- W1966940007 modified "2023-09-27" @default.
- W1966940007 title "Identification of the mass-silent post-transcriptionally modified nucleoside pseudouridine in RNA by matrix-assisted laser desorption/ionization mass spectrometry" @default.
- W1966940007 cites W1435432142 @default.
- W1966940007 cites W1927253718 @default.
- W1966940007 cites W1982947652 @default.
- W1966940007 cites W1991154121 @default.
- W1966940007 cites W1995898053 @default.
- W1966940007 cites W2005193926 @default.
- W1966940007 cites W2017598424 @default.
- W1966940007 cites W2042326593 @default.
- W1966940007 cites W2045913549 @default.
- W1966940007 cites W2047898183 @default.
- W1966940007 cites W2051646379 @default.
- W1966940007 cites W2058123073 @default.
- W1966940007 cites W2072864428 @default.
- W1966940007 cites W2078754103 @default.
- W1966940007 cites W2083553401 @default.
- W1966940007 cites W2096520577 @default.
- W1966940007 cites W2105176936 @default.
- W1966940007 cites W2109446129 @default.
- W1966940007 cites W2139691165 @default.
- W1966940007 cites W2156565222 @default.
- W1966940007 cites W2162906563 @default.
- W1966940007 doi "https://doi.org/10.1093/nar/29.10.e49" @default.
- W1966940007 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/55470" @default.
- W1966940007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11353094" @default.
- W1966940007 hasPublicationYear "2001" @default.
- W1966940007 type Work @default.
- W1966940007 sameAs 1966940007 @default.
- W1966940007 citedByCount "53" @default.
- W1966940007 countsByYear W19669400072012 @default.
- W1966940007 countsByYear W19669400072013 @default.
- W1966940007 countsByYear W19669400072015 @default.
- W1966940007 countsByYear W19669400072016 @default.
- W1966940007 countsByYear W19669400072017 @default.
- W1966940007 countsByYear W19669400072018 @default.
- W1966940007 countsByYear W19669400072019 @default.
- W1966940007 countsByYear W19669400072020 @default.
- W1966940007 countsByYear W19669400072021 @default.
- W1966940007 countsByYear W19669400072022 @default.
- W1966940007 countsByYear W19669400072023 @default.
- W1966940007 crossrefType "journal-article" @default.
- W1966940007 hasAuthorship W1966940007A5072536402 @default.
- W1966940007 hasBestOaLocation W19669400071 @default.
- W1966940007 hasConcept C104317684 @default.
- W1966940007 hasConcept C10879258 @default.
- W1966940007 hasConcept C162356407 @default.
- W1966940007 hasConcept C185592680 @default.
- W1966940007 hasConcept C24107716 @default.
- W1966940007 hasConcept C2779810333 @default.
- W1966940007 hasConcept C2781259782 @default.
- W1966940007 hasConcept C43617362 @default.
- W1966940007 hasConcept C55493867 @default.
- W1966940007 hasConcept C67705224 @default.
- W1966940007 hasConcept C86803240 @default.
- W1966940007 hasConceptScore W1966940007C104317684 @default.
- W1966940007 hasConceptScore W1966940007C10879258 @default.
- W1966940007 hasConceptScore W1966940007C162356407 @default.
- W1966940007 hasConceptScore W1966940007C185592680 @default.
- W1966940007 hasConceptScore W1966940007C24107716 @default.
- W1966940007 hasConceptScore W1966940007C2779810333 @default.
- W1966940007 hasConceptScore W1966940007C2781259782 @default.
- W1966940007 hasConceptScore W1966940007C43617362 @default.
- W1966940007 hasConceptScore W1966940007C55493867 @default.
- W1966940007 hasConceptScore W1966940007C67705224 @default.
- W1966940007 hasConceptScore W1966940007C86803240 @default.
- W1966940007 hasIssue "10" @default.
- W1966940007 hasLocation W19669400071 @default.
- W1966940007 hasLocation W19669400072 @default.
- W1966940007 hasLocation W19669400073 @default.
- W1966940007 hasLocation W19669400074 @default.
- W1966940007 hasOpenAccess W1966940007 @default.
- W1966940007 hasPrimaryLocation W19669400071 @default.
- W1966940007 hasRelatedWork W1963992374 @default.
- W1966940007 hasRelatedWork W1995360205 @default.
- W1966940007 hasRelatedWork W2011103084 @default.
- W1966940007 hasRelatedWork W2065773630 @default.
- W1966940007 hasRelatedWork W2080459625 @default.
- W1966940007 hasRelatedWork W2120276342 @default.
- W1966940007 hasRelatedWork W2153070849 @default.
- W1966940007 hasRelatedWork W2156526915 @default.
- W1966940007 hasRelatedWork W2312636468 @default.
- W1966940007 hasRelatedWork W2528056874 @default.
- W1966940007 hasVolume "29" @default.
- W1966940007 isParatext "false" @default.
- W1966940007 isRetracted "false" @default.
- W1966940007 magId "1966940007" @default.
- W1966940007 workType "article" @default.