FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966941710> ?p ?o ?g. }

• W1966941710 endingPage "1050" @default.
• W1966941710 startingPage "1040" @default.
• W1966941710 abstract "Abstract Foxp3 is a key marker for CD4 + regulatory T cells (T regs ) and was used in developing a multiparameter flow cytometric panel to identify T regs . Achieving reproducible staining and analysis first required optimization of Foxp3 staining. We present a comparative study of PCH101, 236A/E7, 3G3, 206D, 150D, and 259D/C7 clones of anti‐human‐Foxp3 antibodies used in combination with five different fixation/permeabilization buffers. Staining for CD25, CD152, and CD127 was also compared between fixation/permeabilization treatments. Promising antibody/buffer combinations were tested in a panel of peripheral blood mononuclear cells from 10 individuals, and then on fresh versus frozen cells from four individuals. Finally, different fluorochromes coupled to two representative antibodies were compared to optimize separation of Foxp3 + from Foxp3 − events. Foxp3 gates were set using two gating strategies based on CD127 + CD25 − “non‐T regs ” or based on isotype controls. For Foxp3 staining, the best conditions for fixation/permeabilization were obtained using the eBioscience Foxp3, Imgenex, BioLegend, and BD Foxp3 buffers. Comparing results from 10 subjects, 259D/C7, PCH101, 236A/E7, and 206D antibodies yielded statistically higher levels of Foxp3 cells than those by 150D and 3G3 antibodies (mean = 6.9, 5.1, 4.7, and 3.7% compared with 1.7, and 0.3% of CD25 + Foxp3 + events within CD4 + cells, respectively). Importantly, the “nonspecificity” of some antibodies observed with a Foxp3 gate based on isotype controls could be eliminated by setting the Foxp3 gate on “non‐T regs ”. Better separation of Foxp3 + and Foxp3 − populations was observed using the PCH101 clone coupled to Alexa647 compared with FITC or the 259D/C7 clone coupled to PE compared with Alexa488 fluorochrome. Foxp3 staining can be highly variable and depends on the choice of antibody/buffer pair and the fluorochrome used. Selecting the correct population for setting the Foxp3 gate is critical to avoid including non‐T regs in the Foxp3 + gate. The experiments presented here will aid in optimization of flow cytometry staining panels to quantify T reg frequencies in humans. © 2009 International Society for Advancement of Cytometry" @default.
• W1966941710 created "2016-06-24" @default.
• W1966941710 creator A5051080788 @default.
• W1966941710 creator A5065796637 @default.
• W1966941710 creator A5067188451 @default.
• W1966941710 creator A5068659696 @default.
• W1966941710 creator A5079193520 @default.
• W1966941710 creator A5084737140 @default.
• W1966941710 date "2009-10-20" @default.
• W1966941710 modified "2023-10-13" @default.
• W1966941710 title "The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4⁺CD25⁺Foxp3⁺regulatory T cells" @default.
• W1966941710 cites W1560277370 @default.
• W1966941710 cites W1819834770 @default.
• W1966941710 cites W1820160132 @default.
• W1966941710 cites W1965034547 @default.
• W1966941710 cites W1966499706 @default.
• W1966941710 cites W1976999017 @default.
• W1966941710 cites W1984909066 @default.
• W1966941710 cites W1985126564 @default.
• W1966941710 cites W1992037894 @default.
• W1966941710 cites W1996978780 @default.
• W1966941710 cites W1998350666 @default.
• W1966941710 cites W2004708987 @default.
• W1966941710 cites W2011319298 @default.
• W1966941710 cites W2026208522 @default.
• W1966941710 cites W2026836824 @default.
• W1966941710 cites W2048460148 @default.
• W1966941710 cites W2082382293 @default.
• W1966941710 cites W2092015793 @default.
• W1966941710 cites W2093170893 @default.
• W1966941710 cites W2097811455 @default.
• W1966941710 cites W2098481330 @default.
• W1966941710 cites W2108690268 @default.
• W1966941710 cites W2119275893 @default.
• W1966941710 cites W2121059516 @default.
• W1966941710 cites W2130822830 @default.
• W1966941710 cites W2132926599 @default.
• W1966941710 cites W2140608155 @default.
• W1966941710 cites W2141783094 @default.
• W1966941710 cites W2156964052 @default.
• W1966941710 cites W2159346740 @default.
• W1966941710 cites W2160576669 @default.
• W1966941710 cites W4313324526 @default.
• W1966941710 doi "https://doi.org/10.1002/cyto.a.20815" @default.
• W1966941710 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2862733" @default.
• W1966941710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19845018" @default.
• W1966941710 hasPublicationYear "2009" @default.
• W1966941710 type Work @default.
• W1966941710 sameAs 1966941710 @default.
• W1966941710 citedByCount "55" @default.
• W1966941710 countsByYear W19669417102012 @default.
• W1966941710 countsByYear W19669417102013 @default.
• W1966941710 countsByYear W19669417102014 @default.
• W1966941710 countsByYear W19669417102015 @default.
• W1966941710 countsByYear W19669417102016 @default.
• W1966941710 countsByYear W19669417102017 @default.
• W1966941710 countsByYear W19669417102018 @default.
• W1966941710 countsByYear W19669417102019 @default.
• W1966941710 countsByYear W19669417102020 @default.
• W1966941710 countsByYear W19669417102021 @default.
• W1966941710 countsByYear W19669417102022 @default.
• W1966941710 countsByYear W19669417102023 @default.
• W1966941710 crossrefType "journal-article" @default.
• W1966941710 hasAuthorship W1966941710A5051080788 @default.
• W1966941710 hasAuthorship W1966941710A5065796637 @default.
• W1966941710 hasAuthorship W1966941710A5067188451 @default.
• W1966941710 hasAuthorship W1966941710A5068659696 @default.
• W1966941710 hasAuthorship W1966941710A5079193520 @default.
• W1966941710 hasAuthorship W1966941710A5084737140 @default.
• W1966941710 hasBestOaLocation W19669417102 @default.
• W1966941710 hasConcept C153911025 @default.
• W1966941710 hasConcept C159654299 @default.
• W1966941710 hasConcept C185592680 @default.
• W1966941710 hasConcept C203014093 @default.
• W1966941710 hasConcept C2776090121 @default.
• W1966941710 hasConcept C2779727006 @default.
• W1966941710 hasConcept C54355233 @default.
• W1966941710 hasConcept C553184892 @default.
• W1966941710 hasConcept C74864618 @default.
• W1966941710 hasConcept C79484868 @default.
• W1966941710 hasConcept C86803240 @default.
• W1966941710 hasConcept C8891405 @default.
• W1966941710 hasConceptScore W1966941710C153911025 @default.
• W1966941710 hasConceptScore W1966941710C159654299 @default.
• W1966941710 hasConceptScore W1966941710C185592680 @default.
• W1966941710 hasConceptScore W1966941710C203014093 @default.
• W1966941710 hasConceptScore W1966941710C2776090121 @default.
• W1966941710 hasConceptScore W1966941710C2779727006 @default.
• W1966941710 hasConceptScore W1966941710C54355233 @default.
• W1966941710 hasConceptScore W1966941710C553184892 @default.
• W1966941710 hasConceptScore W1966941710C74864618 @default.
• W1966941710 hasConceptScore W1966941710C79484868 @default.
• W1966941710 hasConceptScore W1966941710C86803240 @default.
• W1966941710 hasConceptScore W1966941710C8891405 @default.
• W1966941710 hasIssue "12" @default.
• W1966941710 hasLocation W19669417101 @default.
• W1966941710 hasLocation W19669417102 @default.
• W1966941710 hasLocation W19669417103 @default.

• next