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• W1966968661 abstract "Abstract We present a numerical and experimental study of the effects of ionic strength on electrophoretic focusing and separations. We review the development of ionic strength models for electrophoretic mobility and chemical activity and highlight their differences in the context of electrophoretic separation and focusing simulations. We couple a fast numerical solver for electrophoretic transport with the Onsager–Fuoss model for actual ionic mobility and the extended Debye–Huckle theory for correction of ionic activity. Model predictions for fluorescein mobility as a function of ionic strength and pH compare well with data from CZE experiments. Simulation predictions of preconcentration factors in peak mode ITP also compare well with the published experimental data. We performed ITP experiments to study the effect of ionic strength on the simultaneous focusing and separation. Our comparisons of the latter data with simulation results at 10 and 250 mM ionic strength show the model is able to capture the observed qualitative differences in ITP analyte zone shape and order. Finally, we present simulations of CZE experiments where changes in the ionic strength result in significant change in selectivity and order of analyte peaks. Our simulations of ionic strength effects in capillary electrophoresis compare well with the published experimental data." @default.
• W1966968661 created "2016-06-24" @default.
• W1966968661 creator A5028307856 @default.
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• W1966968661 date "2010-02-26" @default.
• W1966968661 modified "2023-10-17" @default.
• W1966968661 title "Ionic strength effects on electrophoretic focusing and separations" @default.
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• W1966968661 doi "https://doi.org/10.1002/elps.200900560" @default.
• W1966968661 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20191554" @default.
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