FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966976823> ?p ?o ?g. }

• W1966976823 endingPage "465" @default.
• W1966976823 startingPage "461" @default.
• W1966976823 abstract "Within the early region of bacteriophage T7 three genes, 0.3, 1 and 1.3, are most efficiently expressed. They belong to the strongest initiation signals of Escherichia coli. In the T7 wild-type situation the proteins are produced with a molar ratio of gene 1:1.3:0.3 protein = 1:3.9:9.7. DNA fragments of about 30 base pairs comprising the ribosomal binding sites (RBS) of these genes were synthesized and cloned into derivatives of the pDS1 vector ribosomal binding sites (RBS) of these genes were synthesized and cloned into two derivatives of the pDS1 vector just upstream of the mouse dihydrofolate reductase gene. Although all tested RBS fragments contained an initiation triplet, a Shine-Dalgarno sequence and some nucleotides upstream and downstream of this region, only the gene 1.3 RBS fragment showed high efficiency whereas those of genes 0.3 and 1 were at the border of significance. The amount of synthesized mRNA was about the same for all three constructs. A major influence of vector-derived sequences on the RBS activity could be ruled out. The high translational activity of the short 1.3 gene RBS seems to be largely due to its primary structure. The other two RBSs studied require much longer sequences for high activity." @default.
• W1966976823 created "2016-06-24" @default.
• W1966976823 creator A5007073291 @default.
• W1966976823 creator A5011221323 @default.
• W1966976823 creator A5023071452 @default.
• W1966976823 date "1988-08-01" @default.
• W1966976823 modified "2023-09-27" @default.
• W1966976823 title "Only one out of the three strong ribosomal binding sites of the early region of bacteriophage T7 exhibits high translational efficiency in fragments of about 30 base pairs" @default.
• W1966976823 cites W116193106 @default.
• W1966976823 cites W1480177230 @default.
• W1966976823 cites W1667731728 @default.
• W1966976823 cites W1929550892 @default.
• W1966976823 cites W1965212524 @default.
• W1966976823 cites W1969129456 @default.
• W1966976823 cites W1975907920 @default.
• W1966976823 cites W1980279189 @default.
• W1966976823 cites W1987309502 @default.
• W1966976823 cites W2002841189 @default.
• W1966976823 cites W2011936698 @default.
• W1966976823 cites W2013977212 @default.
• W1966976823 cites W2017371535 @default.
• W1966976823 cites W2025763720 @default.
• W1966976823 cites W2027055740 @default.
• W1966976823 cites W2032009967 @default.
• W1966976823 cites W2032754350 @default.
• W1966976823 cites W2034277598 @default.
• W1966976823 cites W2038386417 @default.
• W1966976823 cites W2039666052 @default.
• W1966976823 cites W2043282277 @default.
• W1966976823 cites W2055478501 @default.
• W1966976823 cites W2069149031 @default.
• W1966976823 cites W2079290391 @default.
• W1966976823 cites W2100837269 @default.
• W1966976823 cites W2123668439 @default.
• W1966976823 cites W2154632747 @default.
• W1966976823 cites W3207785987 @default.
• W1966976823 cites W326716566 @default.
• W1966976823 cites W4254839445 @default.
• W1966976823 doi "https://doi.org/10.1111/j.1432-1033.1988.tb14217.x" @default.
• W1966976823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3044788" @default.
• W1966976823 hasPublicationYear "1988" @default.
• W1966976823 type Work @default.
• W1966976823 sameAs 1966976823 @default.
• W1966976823 citedByCount "5" @default.
• W1966976823 crossrefType "journal-article" @default.
• W1966976823 hasAuthorship W1966976823A5007073291 @default.
• W1966976823 hasAuthorship W1966976823A5011221323 @default.
• W1966976823 hasAuthorship W1966976823A5023071452 @default.
• W1966976823 hasBestOaLocation W19669768231 @default.
• W1966976823 hasConcept C104317684 @default.
• W1966976823 hasConcept C153911025 @default.
• W1966976823 hasConcept C185581394 @default.
• W1966976823 hasConcept C2776441376 @default.
• W1966976823 hasConcept C38062823 @default.
• W1966976823 hasConcept C54355233 @default.
• W1966976823 hasConcept C547475151 @default.
• W1966976823 hasConcept C67705224 @default.
• W1966976823 hasConcept C67905577 @default.
• W1966976823 hasConcept C86803240 @default.
• W1966976823 hasConcept C88478588 @default.
• W1966976823 hasConceptScore W1966976823C104317684 @default.
• W1966976823 hasConceptScore W1966976823C153911025 @default.
• W1966976823 hasConceptScore W1966976823C185581394 @default.
• W1966976823 hasConceptScore W1966976823C2776441376 @default.
• W1966976823 hasConceptScore W1966976823C38062823 @default.
• W1966976823 hasConceptScore W1966976823C54355233 @default.
• W1966976823 hasConceptScore W1966976823C547475151 @default.
• W1966976823 hasConceptScore W1966976823C67705224 @default.
• W1966976823 hasConceptScore W1966976823C67905577 @default.
• W1966976823 hasConceptScore W1966976823C86803240 @default.
• W1966976823 hasConceptScore W1966976823C88478588 @default.
• W1966976823 hasIssue "3" @default.
• W1966976823 hasLocation W19669768231 @default.
• W1966976823 hasLocation W19669768232 @default.
• W1966976823 hasOpenAccess W1966976823 @default.
• W1966976823 hasPrimaryLocation W19669768231 @default.
• W1966976823 hasRelatedWork W1863380290 @default.
• W1966976823 hasRelatedWork W1978256988 @default.
• W1966976823 hasRelatedWork W1981142562 @default.
• W1966976823 hasRelatedWork W1999737214 @default.
• W1966976823 hasRelatedWork W2042283535 @default.
• W1966976823 hasRelatedWork W2067243745 @default.
• W1966976823 hasRelatedWork W2073868558 @default.
• W1966976823 hasRelatedWork W2088063203 @default.
• W1966976823 hasRelatedWork W2206447955 @default.
• W1966976823 hasRelatedWork W262780512 @default.
• W1966976823 hasVolume "175" @default.
• W1966976823 isParatext "false" @default.
• W1966976823 isRetracted "false" @default.
• W1966976823 magId "1966976823" @default.
• W1966976823 workType "article" @default.