FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966984631> ?p ?o ?g. }

• W1966984631 abstract "Effective in vivo models of breast cancer are crucial for studying the development and progression of the disease in humans. We sought to engineer a novel mouse model of polyomavirus middle T antigen (PyV mT)-mediated mammary tumourigenesis in which inducible expression of this well-characterized viral oncoprotein is coupled to Cre recombinase (TetO-PyV mT-IRES-Cre recombinase or MIC).MIC mice were crossed to the mouse mammary tumour virus (MMTV)-reverse tetracycline transactivator (rtTA) strain to generate cohorts of virgin females carrying one or both transgenes. Experimental (rtTA/MIC) and control (rtTA or MIC) animals were administered 2 mg/mL doxycycline beginning as early as eight weeks of age and monitored for mammary tumour formation, in parallel with un-induced controls of the same genotypes.Of the rtTA/MIC virgin females studied, 90% developed mammary tumour with complete penetrance to all glands in response to doxycycline and a T50 of seven days post-induction, while induced or un-induced controls remained tumour-free after one year of induction. Histological analyses of rtTA/MIC mammary glands and tumour revealed that lesions followed the canonical stepwise progression of PyV mT tumourigenesis, from hyperplasia to mammary intraepithelial neoplasia/adenoma, carcinoma, and invasive carcinoma that metastasizes to the lung; at each of these stages expression of PyV mT and Cre recombinase transgenes was confirmed. Withdrawal of doxycycline from rtTA/MIC mice with end-stage mammary tumours led to rapid regression, yet animals eventually developed PyV mT-expressing and -non-expressing recurrent masses with varied tumour histopathologies.We have successfully created a temporally regulated mouse model of PyV mT-mediated mammary tumourigenesis that can be used to study Cre recombinase-mediated genetic changes simultaneously. While maintaining all of the hallmark features of the well-established constitutive MMTV-PyV mT model, the utility of this strain derives from the linking of PyV mT and Cre recombinase transgenes; mammary epithelial cells are thereby forced to couple PyV mT expression with conditional ablation of a given gene. This transgenic mouse model will be an important research tool for identifying synthetic viable genetic events that enable PyV mT tumours to evolve in the absence of a key signaling pathway." @default.
• W1966984631 created "2016-06-24" @default.
• W1966984631 creator A5018069190 @default.
• W1966984631 creator A5022523658 @default.
• W1966984631 creator A5027061409 @default.
• W1966984631 creator A5030275299 @default.
• W1966984631 creator A5030606510 @default.
• W1966984631 creator A5043734895 @default.
• W1966984631 date "2014-01-23" @default.
• W1966984631 modified "2023-10-07" @default.
• W1966984631 title "Inducible and coupled expression of the polyomavirus middle T antigen and Cre recombinase in transgenic mice: an in vivo model for synthetic viability in mammary tumour progression" @default.
• W1966984631 cites W1500013333 @default.
• W1966984631 cites W1768274470 @default.
• W1966984631 cites W1992873952 @default.
• W1966984631 cites W2022077728 @default.
• W1966984631 cites W2057841149 @default.
• W1966984631 cites W2059256620 @default.
• W1966984631 cites W2079816467 @default.
• W1966984631 cites W2089016552 @default.
• W1966984631 cites W2089258206 @default.
• W1966984631 cites W2100819355 @default.
• W1966984631 cites W2109994415 @default.
• W1966984631 cites W2111449140 @default.
• W1966984631 cites W2111814753 @default.
• W1966984631 cites W2121220704 @default.
• W1966984631 cites W2154387982 @default.
• W1966984631 cites W2156581417 @default.
• W1966984631 cites W2171238209 @default.
• W1966984631 cites W2171646535 @default.
• W1966984631 doi "https://doi.org/10.1186/bcr3603" @default.
• W1966984631 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3978996" @default.
• W1966984631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24457046" @default.
• W1966984631 hasPublicationYear "2014" @default.
• W1966984631 type Work @default.
• W1966984631 sameAs 1966984631 @default.
• W1966984631 citedByCount "18" @default.
• W1966984631 countsByYear W19669846312014 @default.
• W1966984631 countsByYear W19669846312016 @default.
• W1966984631 countsByYear W19669846312017 @default.
• W1966984631 countsByYear W19669846312018 @default.
• W1966984631 countsByYear W19669846312019 @default.
• W1966984631 countsByYear W19669846312020 @default.
• W1966984631 countsByYear W19669846312021 @default.
• W1966984631 countsByYear W19669846312022 @default.
• W1966984631 countsByYear W19669846312023 @default.
• W1966984631 crossrefType "journal-article" @default.
• W1966984631 hasAuthorship W1966984631A5018069190 @default.
• W1966984631 hasAuthorship W1966984631A5022523658 @default.
• W1966984631 hasAuthorship W1966984631A5027061409 @default.
• W1966984631 hasAuthorship W1966984631A5030275299 @default.
• W1966984631 hasAuthorship W1966984631A5030606510 @default.
• W1966984631 hasAuthorship W1966984631A5043734895 @default.
• W1966984631 hasBestOaLocation W19669846311 @default.
• W1966984631 hasConcept C102230213 @default.
• W1966984631 hasConcept C102414288 @default.
• W1966984631 hasConcept C104317684 @default.
• W1966984631 hasConcept C121608353 @default.
• W1966984631 hasConcept C125929170 @default.
• W1966984631 hasConcept C141035611 @default.
• W1966984631 hasConcept C142724271 @default.
• W1966984631 hasConcept C156695909 @default.
• W1966984631 hasConcept C203014093 @default.
• W1966984631 hasConcept C2777179404 @default.
• W1966984631 hasConcept C2778001805 @default.
• W1966984631 hasConcept C501593827 @default.
• W1966984631 hasConcept C502942594 @default.
• W1966984631 hasConcept C530470458 @default.
• W1966984631 hasConcept C54355233 @default.
• W1966984631 hasConcept C71924100 @default.
• W1966984631 hasConcept C86803240 @default.
• W1966984631 hasConceptScore W1966984631C102230213 @default.
• W1966984631 hasConceptScore W1966984631C102414288 @default.
• W1966984631 hasConceptScore W1966984631C104317684 @default.
• W1966984631 hasConceptScore W1966984631C121608353 @default.
• W1966984631 hasConceptScore W1966984631C125929170 @default.
• W1966984631 hasConceptScore W1966984631C141035611 @default.
• W1966984631 hasConceptScore W1966984631C142724271 @default.
• W1966984631 hasConceptScore W1966984631C156695909 @default.
• W1966984631 hasConceptScore W1966984631C203014093 @default.
• W1966984631 hasConceptScore W1966984631C2777179404 @default.
• W1966984631 hasConceptScore W1966984631C2778001805 @default.
• W1966984631 hasConceptScore W1966984631C501593827 @default.
• W1966984631 hasConceptScore W1966984631C502942594 @default.
• W1966984631 hasConceptScore W1966984631C530470458 @default.
• W1966984631 hasConceptScore W1966984631C54355233 @default.
• W1966984631 hasConceptScore W1966984631C71924100 @default.
• W1966984631 hasConceptScore W1966984631C86803240 @default.
• W1966984631 hasIssue "1" @default.
• W1966984631 hasLocation W19669846311 @default.
• W1966984631 hasLocation W19669846312 @default.
• W1966984631 hasLocation W19669846313 @default.
• W1966984631 hasLocation W19669846314 @default.
• W1966984631 hasLocation W19669846315 @default.
• W1966984631 hasOpenAccess W1966984631 @default.
• W1966984631 hasPrimaryLocation W19669846311 @default.
• W1966984631 hasRelatedWork W1976908356 @default.
• W1966984631 hasRelatedWork W1990488096 @default.
• W1966984631 hasRelatedWork W2030084515 @default.
• W1966984631 hasRelatedWork W2032068651 @default.
• W1966984631 hasRelatedWork W2055996927 @default.

• next