FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966993502> ?p ?o ?g. }

• W1966993502 endingPage "92" @default.
• W1966993502 startingPage "87" @default.
• W1966993502 abstract "The Ig fraction of rabbit anti-T3 antibody was injected into the spleens of BALB/c mice. Four days later, the lymphocytes were recovered from their spleens and were fused with cells of the 653 myeloma cell line. Screening of the hybrid colonies was carried out in a T3 RIA system. Positive colonies were those whose supernatant displaced 125I-labeled T3 from its antibody. The positive cultures were recloned and one was injected ip into mice. The crude IgG fraction of the ascites fluid was affinity purified on an affigel-10 column containing a covalently bound rabbit anti-T3-IgG. In order to eliminate possible endogenous T3 contamination during the affinity purification, the column was stripped with a 40% solution of acetonitriie in 0.2 M acetic acid, neutralized, and then the purification proceeded as described. The affinity purified antibody was an IgG2a isotype. This monoclonal antibody (T3-MAAB) displaced labeled T3 from its antibody in an RIA system. It also mimicked T3 in the stimulation of [3H]2-deoxy-D-glucose (2-DOG) uptake in cultured chick embryo heart cells. After 6 h exposure, the dose-response curve of 2-DOG uptake to T3-MAAB was shifted to the left by at least one order of magnitude when compared to the dose-response curve obtained with T3. After 24 h exposure, T3 had the expected additional stimulatory effect that was dependent on neosynthesis of proteins, while T3-MAAB did not. Also at 24 h exposure, T3-MAAB did not stimulate the incorporation of labeled leucine and uridine into the heart cells while T3 at an equivalent concentration did. The MAAB activity could be abolished by boiling, while boiling did not affect the activity of an equivalent concentration of T3, thus excluding a T3 contamination-mediated effect. We conclude, therefore, that (a) a monoclonal hybridoma producing an antibody that mimics T3 was established; (b) this antibody competed with labeled T3 for an anti-T3 antibody and, like T3, stimulated sugar uptake into cultured chick embryo heart cells; and (c) this antibody, unlike T3, did not stimulate the neosynthesis of proteins." @default.
• W1966993502 created "2016-06-24" @default.
• W1966993502 creator A5002854402 @default.
• W1966993502 creator A5006212041 @default.
• W1966993502 creator A5028711021 @default.
• W1966993502 creator A5052941672 @default.
• W1966993502 creator A5063257343 @default.
• W1966993502 creator A5086103926 @default.
• W1966993502 date "1994-01-01" @default.
• W1966993502 modified "2023-09-27" @default.
• W1966993502 title "The Production of a Monoclonal T3-Antiidiotypic Antibody (T3-MAAB) That Mimics the Effects of T3on 2-Deoxy-D-glucose Uptake in Chick Embryo Heart Cells" @default.
• W1966993502 cites W1968251401 @default.
• W1966993502 cites W2000036320 @default.
• W1966993502 cites W2025272219 @default.
• W1966993502 cites W2093136919 @default.
• W1966993502 doi "https://doi.org/10.1089/thy.1994.4.87" @default.
• W1966993502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8054865" @default.
• W1966993502 hasPublicationYear "1994" @default.
• W1966993502 type Work @default.
• W1966993502 sameAs 1966993502 @default.
• W1966993502 citedByCount "3" @default.
• W1966993502 crossrefType "journal-article" @default.
• W1966993502 hasAuthorship W1966993502A5002854402 @default.
• W1966993502 hasAuthorship W1966993502A5006212041 @default.
• W1966993502 hasAuthorship W1966993502A5028711021 @default.
• W1966993502 hasAuthorship W1966993502A5052941672 @default.
• W1966993502 hasAuthorship W1966993502A5063257343 @default.
• W1966993502 hasAuthorship W1966993502A5086103926 @default.
• W1966993502 hasConcept C134018914 @default.
• W1966993502 hasConcept C153911025 @default.
• W1966993502 hasConcept C159654299 @default.
• W1966993502 hasConcept C185592680 @default.
• W1966993502 hasConcept C196843134 @default.
• W1966993502 hasConcept C203014093 @default.
• W1966993502 hasConcept C24998067 @default.
• W1966993502 hasConcept C542903549 @default.
• W1966993502 hasConcept C55493867 @default.
• W1966993502 hasConcept C86803240 @default.
• W1966993502 hasConcept C95444343 @default.
• W1966993502 hasConceptScore W1966993502C134018914 @default.
• W1966993502 hasConceptScore W1966993502C153911025 @default.
• W1966993502 hasConceptScore W1966993502C159654299 @default.
• W1966993502 hasConceptScore W1966993502C185592680 @default.
• W1966993502 hasConceptScore W1966993502C196843134 @default.
• W1966993502 hasConceptScore W1966993502C203014093 @default.
• W1966993502 hasConceptScore W1966993502C24998067 @default.
• W1966993502 hasConceptScore W1966993502C542903549 @default.
• W1966993502 hasConceptScore W1966993502C55493867 @default.
• W1966993502 hasConceptScore W1966993502C86803240 @default.
• W1966993502 hasConceptScore W1966993502C95444343 @default.
• W1966993502 hasIssue "1" @default.
• W1966993502 hasLocation W19669935021 @default.
• W1966993502 hasLocation W19669935022 @default.
• W1966993502 hasOpenAccess W1966993502 @default.
• W1966993502 hasPrimaryLocation W19669935021 @default.
• W1966993502 hasRelatedWork W1892544028 @default.
• W1966993502 hasRelatedWork W1999686187 @default.
• W1966993502 hasRelatedWork W2005627939 @default.
• W1966993502 hasRelatedWork W2138547054 @default.
• W1966993502 hasRelatedWork W2169340257 @default.
• W1966993502 hasRelatedWork W2368460920 @default.
• W1966993502 hasRelatedWork W2380360044 @default.
• W1966993502 hasRelatedWork W2509789438 @default.
• W1966993502 hasRelatedWork W2596000028 @default.
• W1966993502 hasRelatedWork W2596232480 @default.
• W1966993502 hasVolume "4" @default.
• W1966993502 isParatext "false" @default.
• W1966993502 isRetracted "false" @default.
• W1966993502 magId "1966993502" @default.
• W1966993502 workType "article" @default.