Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967087987> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1967087987 endingPage "321" @default.
- W1967087987 startingPage "313" @default.
- W1967087987 abstract "Heat shock protein 90α (Hsp90α) was immobilized on aminopropyl silica via the N terminus to create the Hsp90α(NT) column or via the C terminus to create the Hsp90α(CT) column. Binding to the exposed C terminus on the Hsp90α(NT) column was characterized using frontal chromatography and the C-terminus ligands coumermycin A1 (CA1) and novobiocin (NOVO). The calculated Kd values were 220 ± 110 nM (CA1) and 100 ± 20 nM (NOVO). Nonlinear chromatography was used to determine the association and dissociation rate constants associated with the NOVO–Hsp90α complex: 22.2 ± 8.8 μM−1 s−1 and 2.7 ± 0.6 s−1, respectively. Binding to the exposed N terminus on the Hsp90α(CT) column was characterized using frontal chromatography. The Kd values of the N-terminus ligands geldanamycin (GM, 90 ± 50 nM), 17-allylamino-17-demethoxygeldanamycin (17-AAG, 210 ± 50 nM), and radicicol (RAD, 20 ± 9 nM) were consistent with previously reported values. The effect of the immobilization on ATPase activity was investigated through the determination of IC50 values for inhibition of ATPase activity on the Hsp90α(CT) column. The IC50 for GM was 2.80 ± 0.18 μM, and the relative IC50 values were 17-AAG > GM > RAD, in agreement with previously reported values and indicating that immobilization had not affected ATPase activity or sensitivity to inhibition." @default.
- W1967087987 created "2016-06-24" @default.
- W1967087987 creator A5011459975 @default.
- W1967087987 creator A5017418601 @default.
- W1967087987 creator A5073267357 @default.
- W1967087987 creator A5075258877 @default.
- W1967087987 creator A5087383129 @default.
- W1967087987 date "2008-02-01" @default.
- W1967087987 modified "2023-09-23" @default.
- W1967087987 title "Initial synthesis and characterization of an immobilized heat shock protein 90 column for online determination of binding affinities" @default.
- W1967087987 cites W1965787931 @default.
- W1967087987 cites W1971578259 @default.
- W1967087987 cites W1977637254 @default.
- W1967087987 cites W1991426846 @default.
- W1967087987 cites W1995997063 @default.
- W1967087987 cites W2000929246 @default.
- W1967087987 cites W2014460006 @default.
- W1967087987 cites W2021246600 @default.
- W1967087987 cites W2022531767 @default.
- W1967087987 cites W2042699875 @default.
- W1967087987 cites W2063956958 @default.
- W1967087987 cites W2074631079 @default.
- W1967087987 cites W2076762409 @default.
- W1967087987 cites W2082432172 @default.
- W1967087987 cites W2089692302 @default.
- W1967087987 cites W2136395938 @default.
- W1967087987 cites W2137372598 @default.
- W1967087987 cites W2157677727 @default.
- W1967087987 doi "https://doi.org/10.1016/j.ab.2007.11.001" @default.
- W1967087987 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2251629" @default.
- W1967087987 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18047824" @default.
- W1967087987 hasPublicationYear "2008" @default.
- W1967087987 type Work @default.
- W1967087987 sameAs 1967087987 @default.
- W1967087987 citedByCount "22" @default.
- W1967087987 countsByYear W19670879872013 @default.
- W1967087987 countsByYear W19670879872014 @default.
- W1967087987 countsByYear W19670879872015 @default.
- W1967087987 countsByYear W19670879872016 @default.
- W1967087987 countsByYear W19670879872017 @default.
- W1967087987 countsByYear W19670879872018 @default.
- W1967087987 countsByYear W19670879872019 @default.
- W1967087987 countsByYear W19670879872020 @default.
- W1967087987 countsByYear W19670879872021 @default.
- W1967087987 crossrefType "journal-article" @default.
- W1967087987 hasAuthorship W1967087987A5011459975 @default.
- W1967087987 hasAuthorship W1967087987A5017418601 @default.
- W1967087987 hasAuthorship W1967087987A5073267357 @default.
- W1967087987 hasAuthorship W1967087987A5075258877 @default.
- W1967087987 hasAuthorship W1967087987A5087383129 @default.
- W1967087987 hasBestOaLocation W19670879872 @default.
- W1967087987 hasConcept C104317684 @default.
- W1967087987 hasConcept C170493617 @default.
- W1967087987 hasConcept C179247698 @default.
- W1967087987 hasConcept C181199279 @default.
- W1967087987 hasConcept C185592680 @default.
- W1967087987 hasConcept C205260736 @default.
- W1967087987 hasConcept C2775932338 @default.
- W1967087987 hasConcept C2780297055 @default.
- W1967087987 hasConcept C43617362 @default.
- W1967087987 hasConcept C51724486 @default.
- W1967087987 hasConcept C55493867 @default.
- W1967087987 hasConcept C74998103 @default.
- W1967087987 hasConceptScore W1967087987C104317684 @default.
- W1967087987 hasConceptScore W1967087987C170493617 @default.
- W1967087987 hasConceptScore W1967087987C179247698 @default.
- W1967087987 hasConceptScore W1967087987C181199279 @default.
- W1967087987 hasConceptScore W1967087987C185592680 @default.
- W1967087987 hasConceptScore W1967087987C205260736 @default.
- W1967087987 hasConceptScore W1967087987C2775932338 @default.
- W1967087987 hasConceptScore W1967087987C2780297055 @default.
- W1967087987 hasConceptScore W1967087987C43617362 @default.
- W1967087987 hasConceptScore W1967087987C51724486 @default.
- W1967087987 hasConceptScore W1967087987C55493867 @default.
- W1967087987 hasConceptScore W1967087987C74998103 @default.
- W1967087987 hasIssue "2" @default.
- W1967087987 hasLocation W19670879871 @default.
- W1967087987 hasLocation W19670879872 @default.
- W1967087987 hasLocation W19670879873 @default.
- W1967087987 hasLocation W19670879874 @default.
- W1967087987 hasOpenAccess W1967087987 @default.
- W1967087987 hasPrimaryLocation W19670879871 @default.
- W1967087987 hasRelatedWork W1978844022 @default.
- W1967087987 hasRelatedWork W199669094 @default.
- W1967087987 hasRelatedWork W2031875389 @default.
- W1967087987 hasRelatedWork W2046579905 @default.
- W1967087987 hasRelatedWork W2078513238 @default.
- W1967087987 hasRelatedWork W2095718201 @default.
- W1967087987 hasRelatedWork W2132702191 @default.
- W1967087987 hasRelatedWork W2147821530 @default.
- W1967087987 hasRelatedWork W2414583721 @default.
- W1967087987 hasRelatedWork W2462775037 @default.
- W1967087987 hasVolume "373" @default.
- W1967087987 isParatext "false" @default.
- W1967087987 isRetracted "false" @default.
- W1967087987 magId "1967087987" @default.
- W1967087987 workType "article" @default.