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- W1967160858 abstract "A new process for preparing oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly on to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. The mixed organic/inorganic thin films have also been extended for use arraying DNA duplex probes, and therefore represent a viable general approach to DNA-based bioarrays. Ideas for interfacing mixed organic/inorganic interfaces to other bioapplications are also discussed." @default.
- W1967160858 created "2016-06-24" @default.
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- W1967160858 date "2005-03-09" @default.
- W1967160858 modified "2023-10-10" @default.
- W1967160858 title "Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays" @default.
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- W1967160858 doi "https://doi.org/10.1002/chem.200400960" @default.
- W1967160858 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15669062" @default.
- W1967160858 hasPublicationYear "2005" @default.
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