FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967188085> ?p ?o ?g. }

• W1967188085 endingPage "194" @default.
• W1967188085 startingPage "189" @default.
• W1967188085 abstract "Studies concerning the localization of immune complexes in lymphoid follicles and the involvement of these trapped immune complexes in the regulation of the immune response have thus far been performed with poorly defined complexes in terms of size and composition. For that reason, the minimum requirements for trapping in terms of number of antigen- and antibody molecules present in immune complexes could not be determined. We here describe the production and in vivo use of a monomeric HSA-HRP antigen-enzyme conjugate, readily demonstrable in cryostat sections and ELISA. This conjugate was obtained by combining the glutaraldehyde coupling-method with chromatography to fractionate monomeric and multimeric constituents. SDS-PAGE analysis showed that the conjugate consisted of a single molecular species of 109 kDa, whereas the often used periodate oxidation coupling method yielded a heterogeneous population of multimeric, oligomeric and monomeric molecules. We investigated the minimal size requirements for the composition of immune complexes to be trapped in murine spleen follicles using three different conjugates (monomeric HSA-HRP, multimeric HSA-HRP and multimeric HSA-HRP-Penicillin) and a panel of anti-HSA and anti-Penicillin monoclonal antibodies. We demonstrate that the smallest immune complexes, consisting of one antibody and two conjugate molecules, do not localize in splenic follicles. Immune complexes prepared with a single monoclonal antibody localize in follicles only if the epitope recognized occurs repeatedly on the antigen. The relevance of these results for physiological follicular trapping of protein antigens is discussed.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W1967188085 created "2016-06-24" @default.
• W1967188085 creator A5002905969 @default.
• W1967188085 creator A5012392552 @default.
• W1967188085 creator A5031482925 @default.
• W1967188085 creator A5079003683 @default.
• W1967188085 creator A5090327278 @default.
• W1967188085 date "1992-02-01" @default.
• W1967188085 modified "2023-09-27" @default.
• W1967188085 title "Production of monomeric antigen-enzyme conjugate to study requirements for follicular immune complex trapping" @default.
• W1967188085 cites W112859519 @default.
• W1967188085 cites W1499746272 @default.
• W1967188085 cites W1526389706 @default.
• W1967188085 cites W1596459311 @default.
• W1967188085 cites W1598778681 @default.
• W1967188085 cites W1773922642 @default.
• W1967188085 cites W1968345663 @default.
• W1967188085 cites W1980082872 @default.
• W1967188085 cites W1981293790 @default.
• W1967188085 cites W1988003238 @default.
• W1967188085 cites W2001789796 @default.
• W1967188085 cites W2010930466 @default.
• W1967188085 cites W2018289835 @default.
• W1967188085 cites W2019090717 @default.
• W1967188085 cites W2019239814 @default.
• W1967188085 cites W2041192038 @default.
• W1967188085 cites W2061834444 @default.
• W1967188085 cites W2062082512 @default.
• W1967188085 cites W2069911117 @default.
• W1967188085 cites W2072514657 @default.
• W1967188085 cites W2080726191 @default.
• W1967188085 cites W2086209569 @default.
• W1967188085 cites W2087646321 @default.
• W1967188085 cites W2100837269 @default.
• W1967188085 cites W2112534467 @default.
• W1967188085 cites W2409497896 @default.
• W1967188085 cites W2413635153 @default.
• W1967188085 cites W2413906877 @default.
• W1967188085 cites W74933395 @default.
• W1967188085 doi "https://doi.org/10.1007/bf00267310" @default.
• W1967188085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1373127" @default.
• W1967188085 hasPublicationYear "1992" @default.
• W1967188085 type Work @default.
• W1967188085 sameAs 1967188085 @default.
• W1967188085 citedByCount "3" @default.
• W1967188085 crossrefType "journal-article" @default.
• W1967188085 hasAuthorship W1967188085A5002905969 @default.
• W1967188085 hasAuthorship W1967188085A5012392552 @default.
• W1967188085 hasAuthorship W1967188085A5031482925 @default.
• W1967188085 hasAuthorship W1967188085A5079003683 @default.
• W1967188085 hasAuthorship W1967188085A5090327278 @default.
• W1967188085 hasBestOaLocation W19671880851 @default.
• W1967188085 hasConcept C134306372 @default.
• W1967188085 hasConcept C147483822 @default.
• W1967188085 hasConcept C153911025 @default.
• W1967188085 hasConcept C159654299 @default.
• W1967188085 hasConcept C185592680 @default.
• W1967188085 hasConcept C195616568 @default.
• W1967188085 hasConcept C197336794 @default.
• W1967188085 hasConcept C203014093 @default.
• W1967188085 hasConcept C2908647359 @default.
• W1967188085 hasConcept C33923547 @default.
• W1967188085 hasConcept C542903549 @default.
• W1967188085 hasConcept C55493867 @default.
• W1967188085 hasConcept C71924100 @default.
• W1967188085 hasConcept C86803240 @default.
• W1967188085 hasConcept C8891405 @default.
• W1967188085 hasConcept C99454951 @default.
• W1967188085 hasConceptScore W1967188085C134306372 @default.
• W1967188085 hasConceptScore W1967188085C147483822 @default.
• W1967188085 hasConceptScore W1967188085C153911025 @default.
• W1967188085 hasConceptScore W1967188085C159654299 @default.
• W1967188085 hasConceptScore W1967188085C185592680 @default.
• W1967188085 hasConceptScore W1967188085C195616568 @default.
• W1967188085 hasConceptScore W1967188085C197336794 @default.
• W1967188085 hasConceptScore W1967188085C203014093 @default.
• W1967188085 hasConceptScore W1967188085C2908647359 @default.
• W1967188085 hasConceptScore W1967188085C33923547 @default.
• W1967188085 hasConceptScore W1967188085C542903549 @default.
• W1967188085 hasConceptScore W1967188085C55493867 @default.
• W1967188085 hasConceptScore W1967188085C71924100 @default.
• W1967188085 hasConceptScore W1967188085C86803240 @default.
• W1967188085 hasConceptScore W1967188085C8891405 @default.
• W1967188085 hasConceptScore W1967188085C99454951 @default.
• W1967188085 hasIssue "2" @default.
• W1967188085 hasLocation W19671880851 @default.
• W1967188085 hasLocation W19671880852 @default.
• W1967188085 hasOpenAccess W1967188085 @default.
• W1967188085 hasPrimaryLocation W19671880851 @default.
• W1967188085 hasRelatedWork W1604248381 @default.
• W1967188085 hasRelatedWork W1973692620 @default.
• W1967188085 hasRelatedWork W1989765228 @default.
• W1967188085 hasRelatedWork W1999614011 @default.
• W1967188085 hasRelatedWork W2001280522 @default.
• W1967188085 hasRelatedWork W2041069158 @default.
• W1967188085 hasRelatedWork W2090054751 @default.
• W1967188085 hasRelatedWork W2162389703 @default.
• W1967188085 hasRelatedWork W2400620861 @default.

• next