FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967354703> ?p ?o ?g. }

• W1967354703 abstract "We have previously reported a synergistic effect of 1,25-dihydroxyvitamin D [1,25-(OH)2D] and agents that elevate intracellular cAMP to induce the expression of the C5a complement receptor in U937 cells. In this report we examine the mechanism of this synergy, considering the hypothesis that the steroid hormone works by facilitating what is ultimately a cAMP-dependent process. We show that U937 cells cultured with 1,25-(OH)2D alone before culture with prostaglandin (PGE2) alone will express C5a receptor (an average of 55 +/- 4% of the receptors expressed with continuous exposure of cells to both agents; P less than 0.05). The reverse, PGE2 followed by 1,25-(OH)2D, causes very little receptor induction. This demonstrates the ability of 1,25-(OH)2D to induce changes in the state of the cell, such that activation of cAMP-dependent protein kinase has effects that are otherwise not seen, in other words 1,25-(OH)2D can prime the cell for the subsequent action of the cAMP messenger system. Furthermore, we are able to substitute, during the priming period, the protein synthesis inhibitor cycloheximide (CHX) for 1,25-(OH)2D. Cells cultured for 24 h with CHX will express C5a receptor when cultured for a second 2-day period with PGE2 at about 77 +/- 7% of the amount obtained with simultaneous exposure to 1,25-(OH)2D and PGE2. The CHX effect is time dependent and visible after 2 h. CHX is not synergistic with 1,25-(OH)2D. Other agents that can also substitute for 1,25-(OH)2D, but not for cAMP, in facilitating C5a receptor expression include retinoic acid and ionomycin, but with less potency. The 1,25-(OH)2D and PGE2 synergy is sensitive to the presence of isobutylmethylxanthine, implicating its dependence on the maintained elevation of intracellular cAMP levels. The synergy does not appear to be sensitive to changes in extracellular or intracellular calcium. We conclude from these results that 1,25-(OH)2D may promote the expression of C5a receptor in these cells in a fashion similar to that by which CHX potentiates other genes, i.e. that 1,25-(OH)2D increases levels of the mRNA encoding the C5a receptor. The mechanism of cAMP's subsequent and necessary action in the induction of C5a receptor expression is not yet clear." @default.
• W1967354703 created "2016-06-24" @default.
• W1967354703 creator A5013974495 @default.
• W1967354703 creator A5046334648 @default.
• W1967354703 creator A5070581832 @default.
• W1967354703 date "1988-11-01" @default.
• W1967354703 modified "2023-09-26" @default.
• W1967354703 title "Expression of C5a Anaphylatoxin Receptor in Monoblastic Cells Involves Facilitation of an Adenosine 3′,5′-Monophosphate-Dependent Process*" @default.
• W1967354703 cites W1509371349 @default.
• W1967354703 cites W1510169588 @default.
• W1967354703 cites W1549484794 @default.
• W1967354703 cites W1599353620 @default.
• W1967354703 cites W1605221277 @default.
• W1967354703 cites W1845004335 @default.
• W1967354703 cites W1967116429 @default.
• W1967354703 cites W1976415203 @default.
• W1967354703 cites W1987647241 @default.
• W1967354703 cites W1995551561 @default.
• W1967354703 cites W2016187099 @default.
• W1967354703 cites W2018108516 @default.
• W1967354703 cites W2024068070 @default.
• W1967354703 cites W2031689439 @default.
• W1967354703 cites W2037737845 @default.
• W1967354703 cites W2074563618 @default.
• W1967354703 cites W2074710283 @default.
• W1967354703 cites W2080532845 @default.
• W1967354703 cites W2082198146 @default.
• W1967354703 cites W2104360623 @default.
• W1967354703 cites W2115663586 @default.
• W1967354703 cites W2136806735 @default.
• W1967354703 cites W2164515753 @default.
• W1967354703 cites W2269901971 @default.
• W1967354703 cites W2399615262 @default.
• W1967354703 cites W326173654 @default.
• W1967354703 doi "https://doi.org/10.1210/endo-123-5-2424" @default.
• W1967354703 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2458913" @default.
• W1967354703 hasPublicationYear "1988" @default.
• W1967354703 type Work @default.
• W1967354703 sameAs 1967354703 @default.
• W1967354703 citedByCount "10" @default.
• W1967354703 crossrefType "journal-article" @default.
• W1967354703 hasAuthorship W1967354703A5013974495 @default.
• W1967354703 hasAuthorship W1967354703A5046334648 @default.
• W1967354703 hasAuthorship W1967354703A5070581832 @default.
• W1967354703 hasConcept C100701293 @default.
• W1967354703 hasConcept C126322002 @default.
• W1967354703 hasConcept C134018914 @default.
• W1967354703 hasConcept C153911025 @default.
• W1967354703 hasConcept C163235415 @default.
• W1967354703 hasConcept C170493617 @default.
• W1967354703 hasConcept C184235292 @default.
• W1967354703 hasConcept C185592680 @default.
• W1967354703 hasConcept C2776991684 @default.
• W1967354703 hasConcept C2778128677 @default.
• W1967354703 hasConcept C2780664492 @default.
• W1967354703 hasConcept C3675279 @default.
• W1967354703 hasConcept C54355233 @default.
• W1967354703 hasConcept C59822182 @default.
• W1967354703 hasConcept C71924100 @default.
• W1967354703 hasConcept C79879829 @default.
• W1967354703 hasConcept C81444415 @default.
• W1967354703 hasConcept C81885089 @default.
• W1967354703 hasConcept C86803240 @default.
• W1967354703 hasConcept C95444343 @default.
• W1967354703 hasConcept C97029542 @default.
• W1967354703 hasConceptScore W1967354703C100701293 @default.
• W1967354703 hasConceptScore W1967354703C126322002 @default.
• W1967354703 hasConceptScore W1967354703C134018914 @default.
• W1967354703 hasConceptScore W1967354703C153911025 @default.
• W1967354703 hasConceptScore W1967354703C163235415 @default.
• W1967354703 hasConceptScore W1967354703C170493617 @default.
• W1967354703 hasConceptScore W1967354703C184235292 @default.
• W1967354703 hasConceptScore W1967354703C185592680 @default.
• W1967354703 hasConceptScore W1967354703C2776991684 @default.
• W1967354703 hasConceptScore W1967354703C2778128677 @default.
• W1967354703 hasConceptScore W1967354703C2780664492 @default.
• W1967354703 hasConceptScore W1967354703C3675279 @default.
• W1967354703 hasConceptScore W1967354703C54355233 @default.
• W1967354703 hasConceptScore W1967354703C59822182 @default.
• W1967354703 hasConceptScore W1967354703C71924100 @default.
• W1967354703 hasConceptScore W1967354703C79879829 @default.
• W1967354703 hasConceptScore W1967354703C81444415 @default.
• W1967354703 hasConceptScore W1967354703C81885089 @default.
• W1967354703 hasConceptScore W1967354703C86803240 @default.
• W1967354703 hasConceptScore W1967354703C95444343 @default.
• W1967354703 hasConceptScore W1967354703C97029542 @default.
• W1967354703 hasLocation W19673547031 @default.
• W1967354703 hasLocation W19673547032 @default.
• W1967354703 hasOpenAccess W1967354703 @default.
• W1967354703 hasPrimaryLocation W19673547031 @default.
• W1967354703 hasRelatedWork W1965943749 @default.
• W1967354703 hasRelatedWork W2026105695 @default.
• W1967354703 hasRelatedWork W2034583712 @default.
• W1967354703 hasRelatedWork W2041773762 @default.
• W1967354703 hasRelatedWork W2063619316 @default.
• W1967354703 hasRelatedWork W2074821979 @default.
• W1967354703 hasRelatedWork W2077452181 @default.
• W1967354703 hasRelatedWork W2081554193 @default.
• W1967354703 hasRelatedWork W2090405647 @default.
• W1967354703 hasRelatedWork W2097683886 @default.

• next