Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967407513> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1967407513 endingPage "237" @default.
- W1967407513 startingPage "229" @default.
- W1967407513 abstract "Background information Single molecule-based super-resolution methods have become important tools to study nanoscale structures in cell biology. However, the complexity of multi-colour applications has prevented them from being widely used amongst biologists. Direct stochastic optical reconstruction microscopy (dSTORM) offers a simple way to perform single molecule super-resolution imaging without the need for an activator fluorophore and compatible with many conventionally used fluorophores. The search for the ideal dye pairs suitable for dual-colour dSTORM has been compromised by the fact that fluorophores spectrally apt for dual-colour imaging differ with respect to the optimal buffer conditions required for photoswitching and the generation of prolonged non-fluorescent (OFF) states. Results We present a novel variant of dSTORM that combines advantages of spectral demixing with the buffer compatible blinking properties of red emitting carbocyanine dyes, spectral demixing dSTORM (SD-dSTORM). In contrast to previously published work, SD-dSTORM requires reduced laser power and fewer imaging frames for the faithful reconstruction of super-resolved biological nanostructures. In addition, SD-dSTORM allows the use of commercially available rather than custom-made probes and does not rely on potentially error-prone cross-talk correction, thus allowing reliable co-localisation. Conclusions SD-dSTORM presents a significant advance towards user-friendly single molecule localisation-based super-resolution microscopy combining advantages of state-of-the-art methodologies to perform fast, reliable and efficient multi-colour dSTORM." @default.
- W1967407513 created "2016-06-24" @default.
- W1967407513 creator A5002414796 @default.
- W1967407513 creator A5010631390 @default.
- W1967407513 creator A5032450795 @default.
- W1967407513 creator A5047265098 @default.
- W1967407513 creator A5063416309 @default.
- W1967407513 date "2012-01-20" @default.
- W1967407513 modified "2023-10-10" @default.
- W1967407513 title "Multi-colour direct STORM with red emitting carbocyanines" @default.
- W1967407513 cites W1480262878 @default.
- W1967407513 cites W1969103349 @default.
- W1967407513 cites W1992858116 @default.
- W1967407513 cites W1996375666 @default.
- W1967407513 cites W2007155950 @default.
- W1967407513 cites W2007971802 @default.
- W1967407513 cites W2051365199 @default.
- W1967407513 cites W2051869246 @default.
- W1967407513 cites W2089725545 @default.
- W1967407513 cites W2106316394 @default.
- W1967407513 cites W2113774034 @default.
- W1967407513 cites W2141083815 @default.
- W1967407513 cites W2167002475 @default.
- W1967407513 doi "https://doi.org/10.1111/boc.201100011" @default.
- W1967407513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22187967" @default.
- W1967407513 hasPublicationYear "2012" @default.
- W1967407513 type Work @default.
- W1967407513 sameAs 1967407513 @default.
- W1967407513 citedByCount "112" @default.
- W1967407513 countsByYear W19674075132013 @default.
- W1967407513 countsByYear W19674075132014 @default.
- W1967407513 countsByYear W19674075132015 @default.
- W1967407513 countsByYear W19674075132016 @default.
- W1967407513 countsByYear W19674075132017 @default.
- W1967407513 countsByYear W19674075132018 @default.
- W1967407513 countsByYear W19674075132019 @default.
- W1967407513 countsByYear W19674075132020 @default.
- W1967407513 countsByYear W19674075132021 @default.
- W1967407513 countsByYear W19674075132022 @default.
- W1967407513 countsByYear W19674075132023 @default.
- W1967407513 crossrefType "journal-article" @default.
- W1967407513 hasAuthorship W1967407513A5002414796 @default.
- W1967407513 hasAuthorship W1967407513A5010631390 @default.
- W1967407513 hasAuthorship W1967407513A5032450795 @default.
- W1967407513 hasAuthorship W1967407513A5047265098 @default.
- W1967407513 hasAuthorship W1967407513A5063416309 @default.
- W1967407513 hasConcept C120665830 @default.
- W1967407513 hasConcept C121332964 @default.
- W1967407513 hasConcept C12554922 @default.
- W1967407513 hasConcept C171250308 @default.
- W1967407513 hasConcept C186060115 @default.
- W1967407513 hasConcept C192562407 @default.
- W1967407513 hasConcept C2778617687 @default.
- W1967407513 hasConcept C86803240 @default.
- W1967407513 hasConcept C91881484 @default.
- W1967407513 hasConceptScore W1967407513C120665830 @default.
- W1967407513 hasConceptScore W1967407513C121332964 @default.
- W1967407513 hasConceptScore W1967407513C12554922 @default.
- W1967407513 hasConceptScore W1967407513C171250308 @default.
- W1967407513 hasConceptScore W1967407513C186060115 @default.
- W1967407513 hasConceptScore W1967407513C192562407 @default.
- W1967407513 hasConceptScore W1967407513C2778617687 @default.
- W1967407513 hasConceptScore W1967407513C86803240 @default.
- W1967407513 hasConceptScore W1967407513C91881484 @default.
- W1967407513 hasIssue "4" @default.
- W1967407513 hasLocation W19674075131 @default.
- W1967407513 hasLocation W19674075132 @default.
- W1967407513 hasOpenAccess W1967407513 @default.
- W1967407513 hasPrimaryLocation W19674075131 @default.
- W1967407513 hasRelatedWork W1973992812 @default.
- W1967407513 hasRelatedWork W2013621820 @default.
- W1967407513 hasRelatedWork W2018914970 @default.
- W1967407513 hasRelatedWork W2022926363 @default.
- W1967407513 hasRelatedWork W2071030197 @default.
- W1967407513 hasRelatedWork W2198056047 @default.
- W1967407513 hasRelatedWork W2297551704 @default.
- W1967407513 hasRelatedWork W2772468872 @default.
- W1967407513 hasRelatedWork W4242560470 @default.
- W1967407513 hasRelatedWork W4302022927 @default.
- W1967407513 hasVolume "104" @default.
- W1967407513 isParatext "false" @default.
- W1967407513 isRetracted "false" @default.
- W1967407513 magId "1967407513" @default.
- W1967407513 workType "article" @default.