Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967543791> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1967543791 endingPage "352" @default.
- W1967543791 startingPage "347" @default.
- W1967543791 abstract "Reassembly experiments, involving isolated human apoproteins A-I and A-II and (dimyristoylglycerophosphocholine)-cholesterol vesicles were performed with apoprotein mixtures at apoprotein A-I/A-II molar ratios varying between 0 and 3. The apoproteins were incubated at 24°C, 28°C and 32°C with either pure dimyristoylglycerophosphocholine vesicles or with dimyristoylglycerophosphocholine cholesterol vesicles containing 0, 5, 10, 15 mol/100 mol cholesterol. The kinetics of association were followed by measuring the increase of the fluorescence polarization ratio after label ing the lipids with diphenyl hexatriene. The complexes were separated from the free protein by gradient ultracentrifugation. Total protein was assayed and the apoproteins A-I and A-II were quantified separately by immunonephelometry. The content of apoprotein A-I was also monitored by measuring the intrinsic tryptophan fluorescence. The results suggest that apoprotein A-II has a greater affinity than apoprotein A-I for the phospholipidcholesterol vesicles and that apoprotein A-II is able to quantitatively displace apoprotein A-I from the lipidprotein complexes. The content of apoprotein A-II in the complexes increases proportionally to the concentration of apoprotein A-II in the incubation mixture until saturation is reached. At saturation the dimyristoylglycerophosphocholine/apoprotein A-II ratio in the complex is dependent upon the cholesterol content of the original vesicles and increases from 60 to 275 mol/mol between 0 and 15 mol/100 mol cholesterol. From these experiments one can calculate that 1 mol human apoprotein A-I is displaced by 2 mol human apoprotein A-II." @default.
- W1967543791 created "2016-06-24" @default.
- W1967543791 creator A5000046652 @default.
- W1967543791 creator A5021746678 @default.
- W1967543791 creator A5038605528 @default.
- W1967543791 creator A5060794153 @default.
- W1967543791 date "1981-07-01" @default.
- W1967543791 modified "2023-09-27" @default.
- W1967543791 title "Displacement of the Human Apoprotein A-I by the Human Apoprotein A-II from Complexes of (Apoprotein A-I)-Phosphatidylcholine-Cholesterol" @default.
- W1967543791 cites W1511938714 @default.
- W1967543791 cites W1544118301 @default.
- W1967543791 cites W1581379253 @default.
- W1967543791 cites W1606635662 @default.
- W1967543791 cites W1775749144 @default.
- W1967543791 cites W1985535231 @default.
- W1967543791 cites W1988220724 @default.
- W1967543791 cites W2010887605 @default.
- W1967543791 cites W2039537157 @default.
- W1967543791 cites W2056153196 @default.
- W1967543791 cites W2113006405 @default.
- W1967543791 cites W2123490257 @default.
- W1967543791 cites W2185157132 @default.
- W1967543791 cites W38373055 @default.
- W1967543791 cites W4250626047 @default.
- W1967543791 doi "https://doi.org/10.1111/j.1432-1033.1981.tb06344.x" @default.
- W1967543791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6791922" @default.
- W1967543791 hasPublicationYear "1981" @default.
- W1967543791 type Work @default.
- W1967543791 sameAs 1967543791 @default.
- W1967543791 citedByCount "35" @default.
- W1967543791 countsByYear W19675437912014 @default.
- W1967543791 crossrefType "journal-article" @default.
- W1967543791 hasAuthorship W1967543791A5000046652 @default.
- W1967543791 hasAuthorship W1967543791A5021746678 @default.
- W1967543791 hasAuthorship W1967543791A5038605528 @default.
- W1967543791 hasAuthorship W1967543791A5060794153 @default.
- W1967543791 hasBestOaLocation W19675437911 @default.
- W1967543791 hasConcept C111357860 @default.
- W1967543791 hasConcept C121332964 @default.
- W1967543791 hasConcept C124815738 @default.
- W1967543791 hasConcept C130316041 @default.
- W1967543791 hasConcept C148898269 @default.
- W1967543791 hasConcept C185592680 @default.
- W1967543791 hasConcept C2776330855 @default.
- W1967543791 hasConcept C2778163477 @default.
- W1967543791 hasConcept C2778918659 @default.
- W1967543791 hasConcept C41625074 @default.
- W1967543791 hasConcept C43617362 @default.
- W1967543791 hasConcept C54689828 @default.
- W1967543791 hasConcept C55493867 @default.
- W1967543791 hasConcept C62520636 @default.
- W1967543791 hasConcept C62746215 @default.
- W1967543791 hasConceptScore W1967543791C111357860 @default.
- W1967543791 hasConceptScore W1967543791C121332964 @default.
- W1967543791 hasConceptScore W1967543791C124815738 @default.
- W1967543791 hasConceptScore W1967543791C130316041 @default.
- W1967543791 hasConceptScore W1967543791C148898269 @default.
- W1967543791 hasConceptScore W1967543791C185592680 @default.
- W1967543791 hasConceptScore W1967543791C2776330855 @default.
- W1967543791 hasConceptScore W1967543791C2778163477 @default.
- W1967543791 hasConceptScore W1967543791C2778918659 @default.
- W1967543791 hasConceptScore W1967543791C41625074 @default.
- W1967543791 hasConceptScore W1967543791C43617362 @default.
- W1967543791 hasConceptScore W1967543791C54689828 @default.
- W1967543791 hasConceptScore W1967543791C55493867 @default.
- W1967543791 hasConceptScore W1967543791C62520636 @default.
- W1967543791 hasConceptScore W1967543791C62746215 @default.
- W1967543791 hasIssue "2" @default.
- W1967543791 hasLocation W19675437911 @default.
- W1967543791 hasLocation W19675437912 @default.
- W1967543791 hasOpenAccess W1967543791 @default.
- W1967543791 hasPrimaryLocation W19675437911 @default.
- W1967543791 hasRelatedWork W1975826912 @default.
- W1967543791 hasRelatedWork W1991542708 @default.
- W1967543791 hasRelatedWork W2011836061 @default.
- W1967543791 hasRelatedWork W2065031942 @default.
- W1967543791 hasRelatedWork W2068607886 @default.
- W1967543791 hasRelatedWork W2410967278 @default.
- W1967543791 hasRelatedWork W2416777797 @default.
- W1967543791 hasRelatedWork W2605719665 @default.
- W1967543791 hasRelatedWork W2646296493 @default.
- W1967543791 hasRelatedWork W68412462 @default.
- W1967543791 hasVolume "117" @default.
- W1967543791 isParatext "false" @default.
- W1967543791 isRetracted "false" @default.
- W1967543791 magId "1967543791" @default.
- W1967543791 workType "article" @default.