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- W1967590905 abstract "Abstract A method for the determination of neutral steroid glucuronides in human urine is described. In most cases, a 10-ml sample of urine was chromatographed on Amberlite XAD-2, then on Amberlyst 15, hydrolysed with Ketodase® and the liberated steroids fractionated on silicic acid columns. The steroids were identified and the specificity of the quantitative determinations verified by gas-liquid chromatography and gas chromatography-mass spectrometry. Values for the excretion of fourteen steroid glucuronides during the follicular and luteal phases of the menstrual cycle are presented. The steroids measured were androsterone, etiocholanolone, dehydroepiandrosterone, 5α-androstane-3α,17α-diol, 5α-androstane-3β,17α-diol, 11β-hydroxyandrosterone, 11 β-hydroxyetiocholanolone, 3α, 16α-dihydroxy-5-androsten-17-one, 5-androstene-3β,16α,17α-triol, 5-androstene-3β,16α,17β-triol, 5β-pregnane-3α,20α-diol, 5β-pregnane-3α,17α,20α-triol, 5-pregnene-3β,17α,20α-triol and tetrahydrocortisone. Statistically significant increases in the excretion of 5β-pregnane-3α,20α-diol, 5β-pregnane-3α,17α,20α-triol and 5-pregnene-3β,17α,20α-triol were observed during the luteal phase. Correlation was demonstrated between the change from the follicular phase to the luteal phase of the menstrual cycle in the excretion ratios of glucuronide-conjugated androsterone /etiocholanolone, 11β-hydroxyandrosterone / 11β-hydroxyetiocholanolone, C 19 steroid diols/11β-hydroxylated 17-ketosteroids and an inverse correlation was observed with the changes in the excretion of C 19 O 2 17-ketosteroids and 5β-pregnane-3α,20α-diol+5β-pregnane-3α,17α,20α-triol. Trivial and systematic nomenclature of steroids: androsterone, 3α-hydroxy-5α-androstan-17-one; etiocholanolone, 3α-hydroxy-5β-androstan-17-one; dehydroepiandrosterone, 3β-hydroxy-5-androsten -i7-one; 11β-hydroxyandrosterone, 3α,11β-dihydroxy-5α-androstan-17-one, 11β-hydroxyetiocholanolone, 3α,11β-dihydroxy-5β-androstan-17-one; progesterone, 4-pregnene-3,20dione; pregnanediol, 5β-pregnane-3α,20α-diol; 17α-hydroxyprogesterone, 17α-hydroxy-4-pregnene 3,20 dione; pregnane-triol, 5β-pregnane-3α,17α,20α-triol; tetrahydrocortisone, 3α,17α,21-tri-hydroxy-5β -pregnane-11, 20-dione." @default.
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- W1967590905 date "1973-12-01" @default.
- W1967590905 modified "2023-09-25" @default.
- W1967590905 title "Urinary excretion of neutral steroid glucuronides with reference to the menstrual cycle" @default.
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- W1967590905 doi "https://doi.org/10.1016/0009-8981(73)90301-x" @default.
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