Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967592012> ?p ?o ?g. }
- W1967592012 endingPage "687" @default.
- W1967592012 startingPage "674" @default.
- W1967592012 abstract "The interactions between polystyrenesulfonate (PSS)/polyallylamine (PAH) multilayers with human serum albumin (HSA) were investigated by means of scanning angle reflectometry (SAR). We find that albumin adsorbs both on multilayers terminating with PSS (negatively charged) or PAH (positively charged) polyelectrolytes. On films terminating with PSS only, an albumin equivalent monolayer is found whereas when PAH constitutes the outer layer, albumin interacts with the multilayer in such a way as to form a protein film that extends over thicknesses that can be as high as four times the largest dimension of the native albumin molecule. Once the protein film is formed, it is found that when the albumin solution is replaced by a pure buffer solution of same ionic strength as the adsorption solution almost no desorption takes place. On the other hand, when a buffer solution of higher ionic strength is brought in contact with the albumin film, a significant amount of adsorbed proteins is released. One also observes that, for albumin solutions of a given protein concentration, the adsorbed protein amount depends on the ionic strength of the adsorption solution. On surfaces terminating with PAH, the adsorbed protein amount first increases rapidly but passes through a maximum and decreases with the ionic strength. The ionic strength corresponding to the maximum of the adsorbed albumin amount itself depends on the albumin concentration. On the other hand, on films terminating with PSS the adsorbed amount increases with the salt concentration before leveling-off. These results show that the underlying complexity of concentration and pH dependent adsorption/desorption equilibria often simply termed protein adsorption is the result of antagonist competing interactions that are mainly of electrostatic origin. We also propose two microscopic models, that are compatible with our experimental observations." @default.
- W1967592012 created "2016-06-24" @default.
- W1967592012 creator A5011061232 @default.
- W1967592012 creator A5025745984 @default.
- W1967592012 creator A5038938912 @default.
- W1967592012 creator A5079520620 @default.
- W1967592012 creator A5079629771 @default.
- W1967592012 creator A5088280008 @default.
- W1967592012 creator A5090113771 @default.
- W1967592012 date "2000-10-21" @default.
- W1967592012 modified "2023-10-04" @default.
- W1967592012 title "Protein Interactions with Polyelectrolyte Multilayers: Interactions between Human Serum Albumin and Polystyrene Sulfonate/Polyallylamine Multilayers" @default.
- W1967592012 cites W1504792186 @default.
- W1967592012 cites W1968083459 @default.
- W1967592012 cites W1976590343 @default.
- W1967592012 cites W1985574773 @default.
- W1967592012 cites W1989492478 @default.
- W1967592012 cites W2000186316 @default.
- W1967592012 cites W2006841815 @default.
- W1967592012 cites W2014386814 @default.
- W1967592012 cites W2021525772 @default.
- W1967592012 cites W2024745799 @default.
- W1967592012 cites W2033728551 @default.
- W1967592012 cites W2046330286 @default.
- W1967592012 cites W2048356991 @default.
- W1967592012 cites W2054272812 @default.
- W1967592012 cites W2056618936 @default.
- W1967592012 cites W2065420518 @default.
- W1967592012 cites W2074780740 @default.
- W1967592012 cites W2080223443 @default.
- W1967592012 cites W2081194040 @default.
- W1967592012 cites W2083017360 @default.
- W1967592012 cites W2088226493 @default.
- W1967592012 cites W2089860029 @default.
- W1967592012 cites W2090718182 @default.
- W1967592012 cites W2117208350 @default.
- W1967592012 cites W2141503847 @default.
- W1967592012 cites W2149272116 @default.
- W1967592012 cites W2155963087 @default.
- W1967592012 cites W2156720951 @default.
- W1967592012 cites W2329764685 @default.
- W1967592012 cites W2609902715 @default.
- W1967592012 cites W4211146992 @default.
- W1967592012 doi "https://doi.org/10.1021/bm005572q" @default.
- W1967592012 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11710198" @default.
- W1967592012 hasPublicationYear "2000" @default.
- W1967592012 type Work @default.
- W1967592012 sameAs 1967592012 @default.
- W1967592012 citedByCount "178" @default.
- W1967592012 countsByYear W19675920122012 @default.
- W1967592012 countsByYear W19675920122013 @default.
- W1967592012 countsByYear W19675920122014 @default.
- W1967592012 countsByYear W19675920122015 @default.
- W1967592012 countsByYear W19675920122016 @default.
- W1967592012 countsByYear W19675920122017 @default.
- W1967592012 countsByYear W19675920122018 @default.
- W1967592012 countsByYear W19675920122019 @default.
- W1967592012 countsByYear W19675920122020 @default.
- W1967592012 countsByYear W19675920122021 @default.
- W1967592012 countsByYear W19675920122022 @default.
- W1967592012 countsByYear W19675920122023 @default.
- W1967592012 crossrefType "journal-article" @default.
- W1967592012 hasAuthorship W1967592012A5011061232 @default.
- W1967592012 hasAuthorship W1967592012A5025745984 @default.
- W1967592012 hasAuthorship W1967592012A5038938912 @default.
- W1967592012 hasAuthorship W1967592012A5079520620 @default.
- W1967592012 hasAuthorship W1967592012A5079629771 @default.
- W1967592012 hasAuthorship W1967592012A5088280008 @default.
- W1967592012 hasAuthorship W1967592012A5090113771 @default.
- W1967592012 hasConcept C127413603 @default.
- W1967592012 hasConcept C150394285 @default.
- W1967592012 hasConcept C153356533 @default.
- W1967592012 hasConcept C162711632 @default.
- W1967592012 hasConcept C165616093 @default.
- W1967592012 hasConcept C170029824 @default.
- W1967592012 hasConcept C178790620 @default.
- W1967592012 hasConcept C184651966 @default.
- W1967592012 hasConcept C185592680 @default.
- W1967592012 hasConcept C187428577 @default.
- W1967592012 hasConcept C2776125364 @default.
- W1967592012 hasConcept C2778674009 @default.
- W1967592012 hasConcept C42360764 @default.
- W1967592012 hasConcept C43617362 @default.
- W1967592012 hasConcept C521977710 @default.
- W1967592012 hasConcept C55493867 @default.
- W1967592012 hasConcept C7070889 @default.
- W1967592012 hasConcept C88001094 @default.
- W1967592012 hasConceptScore W1967592012C127413603 @default.
- W1967592012 hasConceptScore W1967592012C150394285 @default.
- W1967592012 hasConceptScore W1967592012C153356533 @default.
- W1967592012 hasConceptScore W1967592012C162711632 @default.
- W1967592012 hasConceptScore W1967592012C165616093 @default.
- W1967592012 hasConceptScore W1967592012C170029824 @default.
- W1967592012 hasConceptScore W1967592012C178790620 @default.
- W1967592012 hasConceptScore W1967592012C184651966 @default.
- W1967592012 hasConceptScore W1967592012C185592680 @default.
- W1967592012 hasConceptScore W1967592012C187428577 @default.
- W1967592012 hasConceptScore W1967592012C2776125364 @default.