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• W1967649983 endingPage "508" @default.
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• W1967649983 abstract "A method involving high performance liquid chromatography (HPLC) separation associated with tandem mass spectrometry (MS/MS) detection in the multiple reaction monitoring mode was set-up for the measurement of 2-hydroxy-2'-deoxyadenosine (2-OHdAdo). This modified nucleoside, arising from the radical oxidation of 2'deoxyadenosine (dAdo), has been described in the literature as a potential biological marker of the Fenton reaction. Using the specific and sensitive HPLC-MS/MS assay, 8-oxo-7,8-dihydro-2'-deoxyadenosine, 4,6-diamino-5-formamidopyrimidine and 2-hydroxy-2-deoxyadenosine (2-OHdAdo) were measured within 2'-deoxyadenosine and DNA solutions either exposed to gamma-rays or treated under Fenton reaction conditions. It was found that the yield of 2-OHdAdo was low compared to that of 8-oxodAdo under most of the oxidative conditions studied. In particular and in contrast to previous works, the formation of 2-OHdAdo was shown to be a minor process both upon gamma irradiation and under Fenton reaction conditions. However, a significant yield of formation of 2-OHdAdo was observed either upon incubation with high concentrations of Fe2+ ions in the absence of hydrogen peroxide or upon gamma-radiolysis of a nucleoside solution in the presence of the copper/ (o)-phenanthroline complex." @default.
• W1967649983 created "2016-06-24" @default.
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• W1967649983 date "2002-01-01" @default.
• W1967649983 modified "2023-09-26" @default.
• W1967649983 title "Radical Oxidation of the Adenine Moiety of Nucleoside and DNA: 2-Hydroxy-2′-deoxyadenosine is a Minor Decomposition Product" @default.
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• W1967649983 doi "https://doi.org/10.1080/10715760290025889" @default.
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