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- W1967665169 abstract "Abstract Hydrogen peroxide is a major bio‐active molecule produced by mitochondria during aerobic metabolism. In order to monitor that production, platinized ultramicroelectrodes with high surface‐area were used. Their sensitivity was significantly improved following an oxygen plasma treatment. Cyclic voltammetry and chronoamperometry analyses of ferricyanide reduction and hydrogen peroxide oxidation showed that ultramicroelectrodes’ capacitive current was decreased 3‐fold, while faradaic currents remained constant. Then, plasma‐treated ultramicroelectrodes allowed the chronoamperometric detection of 100 nM H 2 O 2 injections. This eventually enabled monitoring the mitochondria release of H 2 O 2 following injection of substrates and a respiratory inhibitor; a flux of few hundreds picomoles H 2 O 2 /mg protein/min was detected." @default.
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- W1967665169 date "2013-01-24" @default.
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- W1967665169 title "Oxygen Plasma Treatment of Platinized Ultramicroelectrodes Increases Sensitivity for Hydrogen Peroxide Detection on Mitochondria" @default.
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- W1967665169 doi "https://doi.org/10.1002/elan.201200409" @default.
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