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• W1967665655 abstract "A solid phase conjugation method is described based on the preadsorption of proteins to aluminium hydroxide adjuvant followed by activation of the adsorbed carrier proteins with iodoacetic acid N-hydroxysuccinimidester or other conjugation reagents. Cysteine-containing peptides were coupled to the iodoacetic acid-activated carrier–adjuvant particles through their SH groups. No dialysis is required since the reaction product is isolated at each step of the procedure by a simple centrifugation and can easily be extensively washed between individual manipulations. The method generates peptide-carrier–adjuvant particles with sterically defined presentation of the peptides at the surface of the particles. When used for immunization of mice and rabbits the conjugates elicited high-titered specific anti-peptide sera, which reacted well with the parent protein in ELISA. The strongest reactions were with the denatured form of the parent protein. On immunoblots antisera to the N- and C-terminus of calreticulin recognized the same Mr 52,000 protein." @default.
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• W1967665655 date "1997-08-01" @default.
• W1967665655 modified "2023-10-18" @default.
• W1967665655 title "Conjugation to preadsorbed preactivated proteins and efficient generation of anti peptide antibodies" @default.
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• W1967665655 doi "https://doi.org/10.1016/s0022-1759(97)00097-5" @default.
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