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- W1967732746 abstract "Bone marrow stromal cell antigen 2 (BST-2, also known as Tetherin) inhibits HIV-1 release and thereby severely impairs viral replication. HIV-1 accessory protein Vpu induces the down-regulation of cell surface BST-2, and counteracts the antiviral function of BST-2. Blocking Vpu-mediated down-regulation of cell surface BST-2 is viewed as a new opportunity for developing anti-HIV drugs. In this study, we have developed a high-throughput cell-based ELISA to identify small molecules that antagonize HIV-1 Vpu function and consequently inhibit HIV-1 replication through rescuing the antiviral activity of host BST-2. This cell-ELISA shows an excellent correlation with results obtained by flow cytometry (FACS). Under optimal conditions, a Z' factor of 0.605 was achieved in a 96-well format. Together, these results demonstrate that this assay can be used to quantify the cell surface level of BST-2 and be adapted to a high-throughput screening for novel anti-HIV compounds." @default.
- W1967732746 created "2016-06-24" @default.
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- W1967732746 date "2011-09-01" @default.
- W1967732746 modified "2023-09-27" @default.
- W1967732746 title "High-throughput assay to identify inhibitors of Vpu-mediated down-regulation of cell surface BST-2" @default.
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- W1967732746 doi "https://doi.org/10.1016/j.antiviral.2011.07.007" @default.
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