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- W1967753602 abstract "Treatment of synchronous cultures of Chlamydomonas reinhardtii with chloramphenicol at 4 hr after the beginning of the light phase led to a preferential loss of 70S ribosomes from the 17,000 × g max supernate. The “lost” 70S ribosomes were found associated with a thylakoid membrane fraction prepared from the 17,000 × g max pellet. Electron microscopic examinations of this fraction revealed that the 70S ribosomes were bound to the unstacked regions of the thylakoid membranes as polygonal penta- and hexamers. These bound ribosomes were only released by treatment with 500 mM KCl and puromycin, suggesting that both ionic interactions and nascent peptide chains were involved in the ribosome-membrane attachment. Since growth of the thylakoid membranes occurs in the light, it is suggested that bound chloroplast ribosomes function in the synthesis of thylakoid membrane proteins." @default.
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- W1967753602 date "1973-05-01" @default.
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- W1967753602 title "Attachment of Chloroplast Polysomes to Thylakoid Membranes in <i>Chlamydomonas reinhardtii</i>" @default.
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- W1967753602 doi "https://doi.org/10.1073/pnas.70.5.1554" @default.
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