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- W1967763973 abstract "UDP-Glc:protein transglucosylase (UPTG) (EC 2.4.1.112), involved in the first step of a two-step mechanism proposed in potato tuber for protein-bound α-glucan synthesis, was found in developing maize endosperm. The enzyme transfers glucosyl residues from UDP-[14C]glucose to an endogenous protein acceptor in a Mn2+-dependent reaction in the absence of added primer. After SDS-polycrylamide gel electrophrresis of the reaction product, radioactivity co-migrated with a 38-kDa protein band. A two-step experiment shows that the developing endosperm of maize contains phosphorylase and starch synthase, capable of utilizing the UPTG reaction product formed during the first step as a primer for the synthesis of protein-bound glucan in the presence of the respective glucosyl donors during the second incubation step." @default.
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- W1967763973 date "1994-01-01" @default.
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- W1967763973 title "UDP-glucose:protein transglucosylase in developing maize endosperm" @default.
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- W1967763973 doi "https://doi.org/10.1016/0168-9452(94)90048-5" @default.
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